人参皂甙单体Rg3对人红白血病细胞株K562细胞增殖及凋亡的影响

目的 探讨人参皂甙单体Rg3对人红白血病细胞株K562细胞增殖及凋亡的影响.方法 将人红白血病细胞株K562细胞传代培养至对数生长期,分组加入不同浓度的人参皂甙单体Rg3,并设置空白对照组.用MTT比色法观察Rg3对体外培养的K562细胞生长的抑制作用;在光学显微镜下观察不同浓度人参皂甙单体Rg3对K562细胞的凋亡作用及计数凋亡率(AR);硝基四氮唑(nitroblue tetrazolium,NBT)还原能力测定试验检测分化指标;DNA琼脂糖凝胶电泳呈现典型的DNA梯形条带图谱.结果 MTT比色法测定显示,人参皂甙单体Rg3作用于K562细胞后可以抑制其增殖并诱导其凋亡,且呈剂量依赖性.结论 人参皂甙单体Rg3能抑制体外培养的K562细胞增殖并诱导其凋亡.

Study on effects of ginsenoside-Rg3 on proliferation and apoptosis of human erythroleukemia cell line K562

Objective To investigate the effects of ginsenoside-Rg3 on proliferation and apoptosis of human erythroleukemia cell line K562.Methods Human erythroleukemia cell line K562 was subcultured to its logarithmic growth phase and was put into ginsenoside-Rg3 of different concentrations.A blank control group was set.The growth inhibition of K562 cell cultured in vitro was evaluated by Using MTT colorimetric assay.Apoptotic K562 cells incubated with ginsenoside-Rg3 of different concentrations were determined by light microscope and the apoptosis rate(AR)was counted.The differentiation index was detected by Nitroblue tetrazolium(NBT)test assay and DNA ladder was revealed through agarose gel electrophoresis analysis.Results The MTT colorimetric assay indicated that ginsenoside-Rg3 could inhibit the growth and also induce the apoptosis of K562 cells in a dose-dependent manner.Conclusion Ginsenoside-Rg3 could had an effect on the inhibition of cell growth and inducement of apoptosis in a K 562 cell line cultured in vitro.