Repair of large osteochondral defects in rabbits using porous hydroxyapatite/collagen (HAp/Col) and fibroblast growth factor‐2 (FGF‐2) |
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| Authors: | Hidetsugu Maehara Shinichi Sotome Toshitaka Yoshii Ichiro Torigoe Yuichi Kawasaki Yumi Sugata Masato Yuasa Masahiro Hirano Naomi Mochizuki Masanori Kikuchi Kenichi Shinomiya Atsushi Okawa |
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| Affiliation: | 1. Department of Orthopaedic and Spinal Surgery, Graduate School, Tokyo Medical and Dental University, 1‐5‐45 Yushima, Bunkyo‐ku, Tokyo 113‐8519, Japan;2. Development Division of Advanced Orthopaedic Therapeutics, Graduate School, Tokyo Medical and Dental University, 1‐5‐45 Yushima, Bunkyo‐ku, Tokyo 113‐8519, Japan;3. Global Center of Excellence (GCOE) Program, International Research Center for Molecular Science in Tooth and Bone Disease, Tokyo Medical and Dental University, 1‐5‐45 Yushima, Bunkyo‐ku, Tokyo 113‐8519, Japan;4. PENTAX New Ceramics Division, HOYA Corporation, 2‐36‐9 Maeno‐cho, Itabashi‐ku, Tokyo 174‐8639, Japan;5. Biomaterial Center, National Institute for Materials Science, 1‐1 Namiki, Tsukuba, Ibaraki 305‐0044, Japan;6. Hard Tissue Genome Research Center, Tokyo Medical and Dental University, 2‐3‐10 Kanda‐Surugadai, Chiyoda‐ku, Tokyo 101‐0062, Japan;7. Core to Core Program for Advanced Bone and Joint Science, Tokyo Medical and Dental University, 2‐3‐10 Kanda‐Surugadai, Chiyoda‐ku, Tokyo 101‐0062, Japan |
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| Abstract: | ![]()
Articular cartilage has a limited capacity for self‐renewal. This article reports the development of a porous hydroxyapatite/collagen (HAp/Col) scaffold as a bone void filler and a vehicle for drug administration. The scaffold consists of HAp nanocrystals and type I atelocollagen. The purpose of this study was to investigate the efficacy of porous HAp/Col impregnated with FGF‐2 to repair large osteochondral defects in a rabbit model. Ninety‐six cylindrical osteochondral defects 5 mm in diameter and 5 mm in depth were created in the femoral trochlear groove of the right knee. Animals were assigned to one of four treatment groups: porous HAp/Col impregnated with 50 µl of FGF‐2 at a concentration of 10 or 100 µg/ml (FGF10 or FGF100 group); porous HAp/Col with 50 µl of PBS (HAp/Col group); and no implantation (defect group). The defect areas were examined grossly and histologically. Subchondral bone regeneration was quantified 3, 6, 12, and 24 weeks after surgery. Abundant bone formation was observed in the HAp/Col implanted groups as compared to the defect group. The FGF10 group displayed not only the most abundant bone regeneration but also the most satisfactory cartilage regeneration, with cartilage presenting a hyaline‐like appearance. These findings suggest that porous HAp/Col with FGF‐2 augments the cartilage repair process. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:677–686, 2010 |
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| Keywords: | osteochondral repair porous hydroxyapatite/collagen fibroblast growth factor‐2 scaffold tissue engineering |
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