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| 三氧化二砷对K562细胞端粒酶活性及P53蛋白表达水平的调节 | |
| 引用本文： | 刘加军,潘祥林,伍新尧,陆惠玲,蔡贵庆. 三氧化二砷对K562细胞端粒酶活性及P53蛋白表达水平的调节[J]. 中国免疫学杂志, 2004, 20(5): 348-351 |
| 作者姓名： | 刘加军 潘祥林 伍新尧 陆惠玲 蔡贵庆 |
| 作者单位： | 1. 中山大学中山医学院法医物证学实验室,广州,510080 2. 山东大学第二医院血液肿瘤中心,济南,250033 |
| 摘 要： | 目的：研究三氧化二砷(As2O3)诱导急性白血病K562细胞的凋亡机制。方法：以不同浓度的As2O3作用于体外培养的K562细胞，检测细胞生长抑制率，观察细胞凋亡时的形态学变化，对细胞端粒酶活性及P53蛋白的表达水平进行检测。结果：As2O3可显著地降低K562细胞端粒酶活性，升高P53蛋白的表达水平，抑制细胞的生长，诱导细胞发生凋亡。并呈现出明显的量-效与时-效关系。结论：As2O3能抑制K562细胞的生长并诱导细胞发生凋亡，降低细胞端粒酶活性及升高P53蛋白的表达水平是其重要作用机制之一。 |
| 关 键 词： | As2O3 端粒酶 细胞凋亡 |
| 文章编号： | 1000-484X(2004)05-0348-04 |
Regulation of arsenic trioxide on telomerase activity and P53 expression in K562 cells |
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| Abstract: | Objective:To investigate the Apoptosis-inducing role of arsenic trioxide on K562 cells.Methods:The variation of both morphology and inhibitory rate of K562 cells were observed in culture medium with various concentrations of arsenic trioxide at different time in vitro.The variation of telomerase activity and P53 protein expression were detected before and after apoptosis occurred.Results:Arsenic trioxide could decrease the telomerase activity and increase P53 protein expression,as well as cause apoptosis and inhibit the growth of K562 cells significantly.The suppression was both in time-and dose-dependent form.Conclusion:Arsenic trioxide can inhibit the growth of K562 cells and induce apoptosis,inhibiting the telomerase activity and increasing the expression of P53 protein may be one of the most important mechanisms. |
| Keywords: | Arsenic trioxide Telomerase Apoptosis |
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