新型钛种植体涂层构建及体外生物安全性评价

目的:构建一种新型钛种植体涂层,检测其表面形貌及体外生物安全性.方法:采用明胶作为交联剂,以本课题组前期制备的包裹SDF-1和rhBMP-2的双层载药纳米微球为基础,通过振荡渗涂交联法将微球交联至微弧氧化涂层的微孔中,在钛种植体表面构建出新型功能性涂层,观察其表面形貌.通过细胞毒性试验、口腔黏膜刺激试验及溶血试验对新型钛种植体涂层体外生物安全性进行初步检测.结果:扫描电镜结果显示新型功能性涂层形貌良好.细胞毒性试验结果显示该涂层100％浸提液细胞毒性为1级,溶血率为4.6％,口腔黏膜刺激试验结果显示该涂层对黏膜无刺激作用.结论:通过振荡渗涂交联法构建的新型钛种植体表面涂层具有良好的表面形貌及生物安全性.

Fabrication and in vitro biological safety of a novel functional coating on microarc-oxidized titanium

Objective:To fabricate and to study the surface morphology and biological safety of a novel coating on microarc-oxidized titanium.Methods:The novel functional coating was fabricated by cross-linking the double-layer nanoparticles loading rhBMP-2 and SDF-1 with gelatin on microarc-oxidation coating on titanium implant surface.The surface topography was observed and optimized,and the biological safety of the novel coating was primarily evaluated by cell toxicity test,oral mucosa stimulation test and hemolysis test in vitro.Results:The novel functional coating possesses excellent morphology.The coating showed the cytotoxicity of score 1 and no mucous membrane irritation,the hemolytic rate of the coating was 4.6％.Conclusion:The coating possesses good morphology and biological safety.