热休克蛋白70的表达与自发性早产合并组织学绒毛膜羊膜炎的关系

目的:探讨热休克蛋白70(HSP70)的表达与自发性早产合并组织学绒毛膜羊膜炎(HCA)的关系。方法:选择2015年6月至2016年1月在郑州大学第三附属医院产科住院并分娩的早产临产孕妇46例为早产临产组,并随机选择同期足月正常分娩的孕妇31例(足月正常组)为对照组。早产临产组孕妇根据分娩后胎膜组织病理学检查结果,分为早产合并HCA组(21例)及早产未合并HCA组(25例)。ELISA法测定并比较各组孕妇外周血、脐血中HSP70蛋白水平,采用免疫组化SP法、Western blotting法及RT-PCR法检测并比较各组孕妇胎膜组织中HSP70蛋白及mRNA的表达水平。采用Pearson相关分析法分析各组HSP70蛋白的相关性。结果:(1)早产合并HCA组孕妇外周血、脐血及胎膜组织中HSP70蛋白的表达和胎膜组织中HSP70 mRNA的表达高于早产未合并HCA组,早产临产组均高于足月正常组,3组间两两比较,差异均有统计学意义(P0.01)。(2)早产合并HCA组中孕妇外周血与脐血及胎膜组织中HSP70蛋白的表达均呈正相关(P0.05)。(3)早产合并HCA组中新生儿败血症发生率高于其余两组(P0.05)。结论:HSP70在早产及其合并HCA时表达增加,HSP70可能参与了早产的发生机制,同时与HCA及新生儿败血症有关。

The Relationship between the Expression of Heat Shock Protein 70 in Preterm Birth with Histologic Chorioamnionitis

Objective:To investigate the relationship between the expression of heat shock protein 70 (HSP70) and preterm birth with histologic chorioamnionitis (HCA).Methods:From June 2015 to January 2016,46 cases of preterm labor(preterm labor group) and 31 cases of full-term delivery(control group) in the Third Affiliated Hospital of Zhengzhou University were included in the study.After delivery,by pathologic analysis of fetal membrane,preterm labor group was classified as two groups.:preterm labor with HCA group(n =21) and preterm labor without HCA group(n =25).The levels of HSP70 protein in maternal peripheral blood were detected by enzyme-linked immunosorbent assay(ELISA).Levels of HSP70 were detected in fetal membrane by immunohistochemistry and Western blotting and real-time PCR.Pearson test was used to analyze the correlation of HSP70 protein in each group.Results:①HSP70 expression in peripheral blood,umbilical cord blood and fetal membranes of preterm labor with HCA group were higher than those of preterm labor without HCA group,HSP70 expression in peripheral blood,umbilical cord blood and fetal membranes of preterm labor group was higher than those of control group,and the difference was statistically significant(P ＜ 0.01).②The levels of HSP70 in maternal serum,umbilical cord blood and fetal membrane in HCA group were positively correlated with each other in the preterm labor with HCA group(P＜0.05).③The incidence of neonatal sepsis in preterm with HCA group was higher than that of the other two groups (P ＜ 0.05).Conclusions:HSP70 expression increases in preterm labor and preterm labor with HCA,which may be involved in the pathogenesis of preterm birth,and is associated with HCA and neonatal sepsis.