STAT-3与Enolase-1在乳腺癌组织中的表达及意义

目的 探讨乳腺癌组织中信号转导及转录活化因子3(STAT-3)和糖酵解酶烯醇化酶(Enolase-1)的表达与雌孕激素受体、淋巴结转移、临床分期、病理分级等的关系.方法 免疫组织化学SP法检测126例乳腺癌和26例乳腺良性病变组织中STAT-3与Enolase-1的表达情况,并分析他们与临床病理参数之间的关系.结果 乳腺癌组织中STAT-3和Enolase-1的阳性表达率分别为82.5％和77.8％,明显高于乳腺良性病变组织(11.5％和7.7％),差异均有统计学意义(x2=42.416,P＜0.05；x2=57.211,P＜0.05)；在有淋巴结转移的乳腺癌组织中阳性表达率分别为85.7％和90.5％,高于无淋巴结转移组,差异均有统计学意义(x2=9.184,P＜0.05；x2=11.014,P＜0.05).相关分析表明STAT-3和Enolase-1的表达呈正相关.在雌激素受体阳性(ER+)和/或孕激素受体阳性(PR+)或表皮生长因子受体2阳性(HER 2+)乳腺癌组织中,有淋巴结转移组中STAT-3和Enolase-1的表达水平均明显高于无淋巴结转移组,差异均有统计学意义(P＜0.05).结论 STAT-3与Enolase-1的表达与乳腺癌的发生发展及侵袭转移相关,且二者有协同作用(r=0.379,P＜0.05).在ER+和(或)PR+或HER-2+乳腺癌组织中,STAT-3和Enolase-1的高表达与淋巴结转移相关.在高表达STAT-3的乳腺癌组织中Enolase-1表达也较高,其联合检测可作为判断乳腺癌恶性程度、评价预后及指导治疗的一项评估指标.

Expression and Clinical Signifiance of STAT-3 and Enolase-1 in Breast Cancer

Objective
To investigate the expression of STAT-3 and Enolase-1 in breast cancer and its relationship with Estrogen receptor status,Progesterone receptor status,lymph node metastasis,clinical stages and histological grading.MethodsThe expression of STAT-3 and Enolase-1 were examined by immunohistochemical streptavid-peroxidase(S-P)assay in 126 cases of breast cancer and 26 cases of benign breast disease.And the significance of its expression was analyzed with clinicopathologic parameters.ResultsThe expression rates of STAT-3(77.8％)and Enolase-1(82.5％)in breast cancer were significantly higher than that in benign breast disease（11.5％ vs.7.7％）and the expression of two proteins were positively correlated(χ²=42.416,P<0.05;χ²=57.211，P<0.05).The expression of STAT-3 and Enolase-1 in breast cancer has no significant difference in age,tumor size,histologic grading and clinical stages.Epression of STAT-3(85.7％)and Enolase-1(90.5％)in breast cancer with metastasis is significantly higher than that without metastasis （χ²=9.184,P<0.05;χ²=11.014,P<0.05）.There was a significant correlation between the high expression of STAT-3 and axillary lymphatic metastasis in comparisons between positive estrogen and/or progesterone receptor expression and Her-2 positive expression（P<0.05）.And there was a significant correlation between the high expression of Enolase-1 and axillary lymphatic metastasis in comparisons with positive estrogen or positive progesterone receptor expression and Her-2 positive expression（P<0.05）.ConclusionBoth STAT-3 and Enolase-1 may be involved in the tumorgenesis development and metastasis of breast cancer.The high expression of STAT-3 and Enolase-1 in estrogen receptor or/and progesterone receptor or Her-2 positive breast cancer was associated with a high possibility of lymph node metastasis.The detection of STAT-3 and Enolase-1 may be helpful in the diagnosis and prognosis of breast cancer.