UF-1000i尿沉渣分析仪假阳性结果分析

目的通过对UF-1000i尿沉渣分析仪假阳性结果进行分析,获得实验室工作中排除假阳性检测的经验和方法。方法采用抽签法随机收取住院患者晨尿样本500份,同时进行UF-1000i尿液沉渣流式自动分析仪(简称UF-1000i)、干化学和显微镜检测(镜检)。UF-1000i检测异常者为阳性,其中UF-1000i检测异常而干化学和镜检正常者为假阳性,将2者检测的有形成分结果进行分析。结果 500份样本中,有178份阳性样本,其中有20份样本UF-1000i的主要检测项目均出现假阳性,其中红细胞(erythrocyte,RBC)、白细胞(leukocyte,WBC)、管型、上皮细胞(epithelial cells,EC)的假阳性率分别为10.8%、8.2%、23.1%、14.3%。结论 UF-1000i尿沉渣分析仪提高了尿液分析的过筛检查实验室检测效率,但对其阳性结果还需密切注意,应该避免假阳性结果。

Analysis of false positive results of Sysmex UF-1000i urine sediment analyzer

Objective To evaluate positive results of UF-1000i Urinary sediment analyzer and analyze false positive results. Methods A total of 500 urine samples were collected randomly and determined by UF-1000i full automatic urinary sediment analyzer(UF-1000i), urine strips and microscopic analysis. positive results of samples were deemed as false when results were abnormal with UF-1000i analyzing but normal with urine strips and microscopic analysis. Two results of urine formed elements were contrasted between UF-1000i and microscopic analysis in samples with false positive results. Results Totally 178 of 500 samples showed positive results with UF-1000i analysis, in which 20 samples were determined as false positive results in main examined items with microscopic analysis. In 178 positive results, the false positive rate of erythrocyte(RBC), leukocyte(WBC), cast and epithelial cells(EC) were 10.8%, 8.2%, 23.1% and 14.3% separately. Conclusion The analyses on urinary sediments by UF-1000i full automatic urinary sediment analyzer was effective. But it is important to observe sediments by microscope to avoid false positive results when results were abnormal with UF-1000i analysis.