纤维蛋白原B β-455G/A多态性与缺血性中风的相关性

目的　研究纤维蛋白原 Bβ( fibrinogen Bβ,FGβ)基因启动子区域 - 4 5 5 G/ A变异与缺血性中风的关系。方法　随机抽取住院高血压缺血性中风患者 86例 (中风组 ) ,高血压非缺血性中风患者 85例(高血压组 ) ,门诊健康查体者 90人 (对照组 ) ,应用聚合酶链反应加 Hae 内切酶检测 FGβ基因启动子区域 - 4 5 5 G/ A的多态性 ,血浆纤维蛋白原水平测定采用凝血酶原时间法。结果　A- 455等位基因在中风组的分布频率较高血压组及对照组明显增高 (分别为 0 .2 2、0 .13、0 .11,χ2 =8.35 ,P<0 .0 5 )。不同组别的基因型的分布不同 ,G/ A、A/ A基因型在中风组更常见 ( χ2 =10 .0 3,P<0 .0 5 )。缺血性中风组血浆纤维蛋白原水平 ( 4 .82± 0 .2 6 )显著高于对照组及高血压组 ( F=5 .98,P<0 .0 1)。在缺血性中风组中及高血压组中- 4 5 5 G/ G基因型的血浆纤维蛋白原水平明显低于 - 4 5 5 G/ A和 - 4 5 5 A/ A的基因型 ( P<0 .0 5 ) ,而在对照组不同基因型的血浆纤维蛋白原水平差异则无显著性。结论　血浆中纤维蛋白原水平受 FGβ基因 -4 5 5 G/ A多态性的影响 ,A- 455等位基因是缺血性中风的一个相对独立的危险因素

Association between the polymorphism of β-fibrinogen gene -455G/A and ischemic stroke

OBJECTIVE: To study the association between the variation of beta fibrinogen gene promoter -455G/A and ischemic stroke. METHODS: Eighty-six hypertensive patients with ischemic stroke (stroke group) and 85 hypertensive patients without ischemic stroke (hypertensive group) were randomly selected from the in patients, and 90 healthy persons(control group) were recruited for this study. Polymerase chain reaction with restrictive enzyme Hae III (PCR-restriction fragment length polymorphism method) was employed to analyze the polymorphism of beta-fibrinogen gene promoter -455G/A. Plasma fibrinogen levels were measured with prothrombin time (PT) assay. RESULTS: A(-455) allele frequencies of the stroke group (0.22) was significantly higher than that of the control group and the hypertensive group (0.11, 0.13, chi-square=8.3 P<0.05). The genotype exhibited significant difference among the 3 groups; G/A and A/A were more commonly seen in the stroke group(chi-square=10.03,P<0.05). The mean fibrinogen level of the stroke group(4.82+/-0.26 g/L) was significantly higher than that of the control group and the hypertensive group(4.37+/-0.19, 5.50+/-0.20, F=5.98 P<0.01) In both stroke group and hypertensive group, the plasma fibrinogen levels in patients with -455G/G were significantly lower than those in patients with -455G/A, and -455A/A but the difference was not significant among the 3 genotypes in the control group. CONCLUSION: Plasma fibrinogen level could be affected by the beta-fibrinogen gene -455G/A polymorphism. And A(-455) allele may be an independent risk factor for ischemic stroke.