| 假杜鹃中总黄酮的含量测定 |
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| 引用本文： | 刘冬珠,朱全红,张宏伟. 假杜鹃中总黄酮的含量测定[J]. 中医药导报, 2009, 15(2) |
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| 作者姓名： | 刘冬珠 朱全红 张宏伟 |
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| 作者单位： | 南方医科大学中医药学院,广东,广州,510515 |
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| 摘 要： | 目的:建立假杜鹃中总黄酮含量的测定方法,并测定其含量.方法:以硝酸铝、亚硝酸钠及氢氧化钠为显色剂,利用紫外分光光度计测定假杜鹃中总黄酮的含量.结果:最佳检测波长为510 nm,芦丁质量浓度在0.0151-0.30897 mg/mL范围内,吸收度与质量浓度呈良好的线性关系,回归方程为y=11.471x+0.005(r=0.9998),测定的平均回收率为RSD%=0.88%,精密度试验RSD%=0.0247%,重复性试验RSD%=2.79%,稳定性实验RSD%=0.13%.结论:利用紫外分光光度法测定假杜鹃中总黄酮的含量,简便快速且有良好的稳定性、准确性和重现性,可用于药材的质量控制.
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| 关 键 词： | 假杜鹃 总黄酮 |
Determination of Total Flavones in Barleria cristata L. |
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| LIU Dong-zhu,ZHU Quan-hong,ZHANG Hong-wei. Determination of Total Flavones in Barleria cristata L.[J]. GUIDING JOURNAL OF TCM, 2009, 15(2) |
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| Authors: | LIU Dong-zhu ZHU Quan-hong ZHANG Hong-wei |
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| Affiliation: | LIU Dong-zhu,ZHU Quan-hong,ZHANG Hong-wei College of Traditional Chinese Medicine,Southern Medical University,Guangzhou,China 510515 |
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| Abstract: | Objective:To establish a method on determination of total flavones in Barleria cristata L.and determination of its content.Methods:With NaNO2-A1(NO3)3-NaOH as chromogenic agent,total flavanone content of Barleria cristata L.is determined by ultraviolet spectrophotometry.Results:The optimal detective wavelength was 510 nm,and absorbability and mass concent ration was satisfactory linear relations hip at the mass concentration of rutin was in 0.0151-0.30897 mg/mL.The regression equation was Y=11.471X+0.005(r=... |
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| Keywords: | Barleria cristata L. Total flavones |
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