高原现场体外动物细胞培养的实验研究

目的：初步探索在高原现场进行体外动物细胞培养的可行性。方法：采用成年家兔肝细胞进行体外原代培养，观察细胞形态及培养上清中ALB和AFP水平变化。结果：细胞接种4h开始贴壁，8h开始增殖，胞内核数目增多，6d已生长满瓶；培养上清中ALB及AFP水平均呈现显著升高，其达峰值时间分别为8d和6d。结论：体外培养肝细胞生长、增殖及其生物学功能良好，提示在本驻地海拔高度条件下能够进行体外动物细胞培养。

Cell culture in vitro at high altitude

Objective: To study the feasibility of cell culture in vitro at high altitude.Methods: The rabbit hepatocytes were cultured at high altitude,and then the changes of cell shape and levels of the biochemical substances in culture supernatants were investigated at the different time.Results: After cultured 4 h,the hepatocytes began to adhibit to the wall of plant board;after 8 h,the hapatocytes began to proliferate and the number of cell nucleus began to increase;after 6 days,the plant board had been suffused with growthing hepatocytes.The levels of alpha -fetoprotein(ALB) and albumin(AFP) increased remarkably and reached to the peak within 8 days and 6 days,respectively.Conclusion: The rabbit hepatocytes can be cultured well at high altitude.