Survivin反义寡核苷酸对肝癌细胞株HEPG2的抑制作用

目的观察survivin反义寡核苷酸（ASODN）对肝癌细胞的增殖、凋亡的影响。方法人工合成survivin硫代ASODN,通过脂质体转染肝癌细胞株HEPG2后,用MTT法检测细胞毒作用,流式细胞术检测细胞的凋亡;RT-PCR、Western blot检测survivin mRNA及蛋白的表达。结果survivin ASODN抑制HEPG2增殖,呈剂量依赖性;各survivin ASODN处理组HEPG2细胞凋亡率显著高于对照组（P〈0.01）;survivin ASODN处理组HEPG2细胞survivin蛋白及mRNA的表达水平显著下降（P〈0.01）。结论survivin ASODN能够抑制HEPG2细胞增殖、诱导凋亡。

Inhibitory Effects of Survivin Antisense Oligonucleotide on Hepatocellular Carcinoma Cell HEPG2

Objective To explore the effect of antisense oligodeoxynucleotide（ASODN） of survivin gene on expression of survivin mRNA,protein and chemotherapy sensitivity of hepatocellular carcinoma cell HepG2.Methods MTT assay was applied to the determination of cell proliferation in HEPG2 cells treated with different concentration survivin antisense compounds.apoptotic index（AI） was examined by flow cytometry;By using RT-PCR and western-blot,the expression levels of survivin mRNA and protein were detected.Results Survivin ASODN inhibited the HEPG2 cells proliferation,the inhibitory rate of different concentration ASODN were 22.0%,41.5%,57.3%,62.7% respectively.the AI of ASODN groups was significantly higher than that of other groups;Survivin ASODN down-regulated survivin expression（mRNA and protein） in a dose dependent manner.Its maximum effect was achieved at a concentration of 800 nmol/L.Conclusion Antisense survivin has significant inhibitory effect on growth of HEPG2 cells through down-regulating survivin mRNA and protein.And antisense oligonudecleotides targeting survivin have a bright prospect for the therapy of hepatocellular carcinoma.