| RGD肽表面修饰壳聚糖载基因纳米粒子的体外实验研究 |
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| 引用本文： | 唐锋,管珩,刘昌伟,李拥军,郑月宏,李晓慧,鲍军波,唐丽娜,宋存先.RGD肽表面修饰壳聚糖载基因纳米粒子的体外实验研究[J].国际生物医学工程杂志,2003,32(1):129-132,前插1. |
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| 作者姓名： | 唐锋 管珩 刘昌伟 李拥军 郑月宏 李晓慧 鲍军波 唐丽娜 宋存先 |
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| 作者单位： | 中国医学科学院北京协和医学院北京协和医院血管外科北京,100032;中国医学科学院北京协和医学院生物医学工程研究所,天津,300192; |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 目的 将Arg-Gly-Asp(RGD)肽偶联到壳聚糖(cH)材料表面,并制备成包载质粒DNA的纳米粒子,以未偶连RGD的壳聚糖载质粒DNA作为对照,进行体外内皮细胞转染,观察其是否能提高对内皮细胞的转染效率.方法 以1-乙基-3-(3-二甲基氨基丙基)碳化二亚胺盐酸盐(EDC)和N-羟基丁二酰亚胺(NHS)为偶联剂,通过酰胺键将RGD肽偶联到壳聚糖表面,对其进行表征,并以未偶连RGD的壳聚糖作为对照,制备载pEGFP-C1质粒DNA纳米粒子,比较2者对Hy926细胞的转染效率.结果 壳聚糖-RGD(CH-RGD)载基因纳米粒子转染Hy926细胞的效率明显高于未偶连RGD的壳聚糖载基因纳米粒子(35.7%vs 14.3%,P<0.001).结论 RGD肽表面修饰壳聚糖载基因纳米粒子可用于体外细胞转染,其对细胞的转染效率明显优于未偶连RGD的壳聚糖.
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| 关 键 词： | RGD肽 壳聚糖 纳米粒子 基因载体 |
In vitro study on RGD-immobllized chitosan nanoparticles as gene vectors |
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| Abstract: | Objective To investigate gene transfection efficiency in cell culture mediated by chitosan Nanoparticles immobilized with Arg-Gly-Asp(arginine-glycine-aspartic acid,RGD)-peptides.Mothods RGD-peptides was immobilized on chitosan by chemical crosslinking. Plasmid pEGFP-Cl was encapsulated into Chitosan-RGD-nanoparticles (Chitosan-RGD-pEGFP-NPs) by complex coacervation method. Chitusan-pEGFP-nanoparticles without RGD were used as the control to evaluate gene tranfection efficiency into Hy926 cells in vitro.Results The transfection efficiency of Chitosan-RGD-pEGFP-NPs is statistically higher than Chitosan-pEGFP-NPs.(35.7% vs 14.3%,P<0.001) Conclusion Chitosan-RGD NPs could act as gene vector to transfect specific gene in vitro and the transfection efficiency is higher than that of non-RGD chitosan nanoparticles. |
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| Keywords: | Arg-Gly-Asp-peptidesChitosanNanoparticlesGene vectors |
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