精子相关抗原9在子宫平滑肌肉瘤中的表达及临床意义

目的:研究子宫平滑肌肉瘤,富于细胞性子宫平滑肌瘤及子宫平滑肌瘤组织中精子相关抗原9(SPAG9)的表达及临床意义。方法:应用免疫组织化学技术检测19例子宫平滑肌肉瘤,22例富于细胞性子宫平滑肌瘤及30例子宫平滑肌瘤组织中SPAG9蛋白的表达。结果:SPAG9在子宫平滑肌肉瘤及富于细胞性子宫平滑肌瘤中表达率均为100%,在子宫平滑肌瘤中的表达率为0。根据Formwitz评分比较:SPAG9蛋白在子宫平滑肌肉瘤组织中的表达高于富于细胞性子宫平滑肌瘤和子宫平滑肌瘤中的表达,差异有统计学意义(P<0.05);子宫平滑肌肉瘤组织的表达中,不同核分裂数组织SPAG9蛋白的表达差异无统计学意义(P>0.05),细胞异型性不同的组织SPAG9蛋白的表达差异有统计学意义(P<0.05)。结论:SPAG9蛋白的异常表达与子宫平滑肌肉瘤的发生及预后相关,有望成为子宫平滑肌肉瘤与富于细胞性子宫平滑肌瘤鉴别诊断的生物学标记。

Expression and clinical significance of sperm associated antigen 9 in the tissue of leiomyosarcoma

Objective:To investigate the expression of sperm associated antigen9(SPAG9)in the tissue of leiomyosarcoma,cellular leiomyoma and uterine leiomyoma and its clinical significance.Methods:Immunohistochemistry staining were used to detect the expression of SPAG9 on the protein level in the tissue obtained from 19 patients with leiomyosarcoma,22 patients with cellular leiomyoma and 30 patients with uterine leiomyoma.Results:In the tissue of leiomyosarcoma,SPAG9 expression rate was 100%,In the cellular leiomyoma the expression rate was 100%,and the expression rate in leiomyoma tissue was 0.The Formwitz score of leiomyosarcoma tissue was higher than cellular leiomyoma and uterine leiomyoma,the difference was statistically significant(P<0.05).In the expression of SPAG9 in leiomyosarcoma,different number of nuclear division tissue have different SPAG9 protein expression,the difference was not statistically significant(P>0.05).Different cellular atypia tissue have different SPAG9 protein expression,and the difference is statistically significant(P<0.05).Conclusion:The abnormal expression of SPAG9 protein in leiomyosarcoma might be related with the occurrence and prognosis of leiomyosarcoma.SPAG9 has the possibility to be the biological marker for differential diagnosis of leiomyosarcoma and cellular leiomyoma.