长链脂肪乳剂预处理对大鼠心肌缺血再灌注损伤的保护作用

目的：探讨长链脂肪乳剂预处理对大鼠心肌缺血再灌注（I/R）损伤的保护作用。方法：成年SD大鼠21只，随机分为3组，每组7只；假手术组（Sham组）：不进行任何药物处理；模型组（Model组）：0.9%氯化钠溶液2 mg·kg-1·d-1静脉注射5 d；长链脂肪乳剂组（LE组）：Intralipid 2 mg·kg-1·d-1静脉注射5 d。末次给药后24 h建立大鼠左前降支结扎I/R模型，其中Sham组只穿线不结扎。持续监测大鼠血流动力学，取心脏进行梗死面积分析，取血检测乳酸脱氢酶（LDH）。结果：与Model组比较，LE组心肌梗死面积占危险区心肌百分比（IR/AAR）和梗死面积占左室面积百分比（IR/LV）显著降低（均P＜0.05），LDH释放量显著降低（P＜0.05）。与Sham组比较，Model组缺血期及再灌注期平均动脉压（MAP）及心率与收缩压的乘积（RPP）均显著降低（均P＜0.05）；而LE组和Sham组缺血期及再灌注期MAP及RPP比较差异均无统计学意义（P＞0.05）。结论：长链脂肪乳剂预处理能够缩小大鼠心肌I/R损伤后梗死面积、减少LDH释放，同时减缓因I/R损伤所致的血流动力学改变。

The effect of long chain fat emulsion on the myocardial ischemia reperfusion injury in rats

Objective: To investigate the protective effect of long chain fat emulsion on myocardial ischemia/reperfusion (I/R) injury in rats. Methods: Twenty-one adult SD rats were randomly divided into three groups (n=7): sham operation group (Sham group), no drug treatment; model group (Model group), normal saline 2 mg·kg-1·d-1 injection for 5 days; long chain fat emulsion group (LE group), intralipid 2 mg·kg-1·d-1 injection for 5 days. Twenty-four hours after the last administration, the model of myocardial ischemia reperfusion (I/R) injury in rats was established by ligating the left anterior descending coronary. Hemodynamics was continuously monitored. The heart was taken for infarction area analysis, and the blood was collected for lactate dehydrogenase (LDH) detection. Results: Compared with the Model group, infarct size divided by area at risk (IR/AAR) and infarct size divided by left ventricle (IR/LV) were significantly reduced in LE group (all P<0.05), and LDH release was markedly lower than that in the Model group (P<0.05). The mean arterial pressure (MAP) and rate-pressure product (RPP) of rats in Model group were statistically lower than Sham group in ischemic period and reperfusion period (all P<0.05), while there was no significant difference between LE group and Sham group (P>0.05). Conclusion: Long chain fat emulsion pretreatment can reduce infarct area after myocardial I/R injury in rats, reduce LDH release and alleviate hemodynamic changes caused by I/R injury.