中国脑型疟患者恶性疟原虫分离株裂殖子表面蛋白2基因的分子克隆及序列分析

目的为设计研制安全有效的人脑型疟疫苗提供科学依据。方法应用多聚酶链反应（ＰＣＲ）对５例中国脑型疟患者恶性疟原虫云南省勐腊县勐罕（ＣＭＨ／ＹＮ）分离株和云南省盈江县农场（ＣＹＪ／ＹＮ）分离株基因组裂殖子表面蛋白２（ＭＳＰ２）基因进行扩增，将扩增产物分别经ＢａｍＨＩ和ＨｉｎｄⅢ双酶切后，回收的ＭＳＰ２基因分子定向克隆Ｍ１３ｍｐ１８和Ｍ１３ｍｐ１９载体，按Ｓａｎｇｅｒ双脱氧链终止法进行ＤＮＡ序列测定，并与恶性疟原虫株ＦＣ２７、Ｋ１、ＩＣ１和ＣＡＭＰ株进行同源性分析比较。结果５例中国脑型疟患者恶性疟原虫ＣＭＨ／ＹＮ和ＣＹＪ／ＹＮ分离株ＭＳＰ２基因之间序列完全相同，全长为８００ｂｐ，编码２６４个氨基酸，中央的串联重复序列含２×３２肽序列，与ＦＣ２７、Ｋ１株之间核苷酸同源性为９８８％，而与ＩＣ１、ＣＡＭＰ株之间核苷酸同源性为２８２％。结论中国脑型疟患者恶性疟原虫分离株ＭＳＰ２基因突变及其表达的异常可能是导致脑型疟昏迷的一种重要的分子病因

The association of HLA B27 binding peptide and spondylarthropathies

Objective To explore the association of spondylarthropathies HAL B27 binding peptide. Methods B27 molecules expressed on the B27 T2 mutant cell line do not have peptides. Such empty B27 molecules were not recognized by peptide dependent monoclonal antibody, B27.M2. Positive B27.M2 reactivity resulted when B27 T2 cells were incubated with peptide RRKAMFEDI derived from Chlamydia hsp60. THe importance of each residue in RRKAMFEDI was analysed by testing analogs in which each of the 9 residues in the peptide was consecutively substituted by 19 other amino acids. Results B27.M2 reactivity was the highest with arginine in second residue. The reactivities of residues other than arginine were less than 30% of the value with the parent peptide. Conclusion The stringency for other residues was relatively low, indicating that immune recognition of B27 peptide complexes might have rather low stringency for peptide sequences. These peptide residues probably influenced antibody reactivity by inducing conformational changes.