Purification and characterization of equine infectious anemia virus |
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| Authors: | H. D. Matheka L. Coggins J. N. Shively N. L. Norcross |
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| Affiliation: | (1) Research Laboratory for Equine Infectious Diseases, Department of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York, USA;(2) Federal Research Institute for Animal Virus Diseases, P.O.Box 1149, D-7400 Tübingen, Federal Republic of Germany |
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| Abstract: | Summary EIA virus was purified from equine fetal kidney cell cultures by PEG-precipitation, two sucrose-gradient sedimentations (5–30 per cent) and (25 to 60 per cent) centrifugation, using the immunodiffusion test to follow the procedure. Purified EIA virus had a density (20° C) of 1.162 and a sedimentation constant of S20W=656. Electron microscopy revealed a particle of about 100 nm in diameter with a very flexible but usually spherical shape. The dense core may be at various locations inside the membrane bound particle.With 6 Figures |
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