沙棘油对小鼠心肌自由基代谢和超微结构的影响

目的 :　观察沙棘油 ( OHR)对运动小鼠血清 GOT、心肌 SOD、MDA及超微结构的影响。方法 :　 70只雄性 ICR小鼠随机分为六组 :安静对照组、安静沙棘组、训练即刻组、训练即刻沙棘组、训练恢复 2 4小时组和训练恢复 2 4小时沙棘组。经过 6周游泳耐力训练后进行一次力竭性游泳 ,观察了各组小鼠血清谷草转氨酶 ( GOT)、心肌超氧化物歧化酶 ( SOD)、丙二醛 ( MDA)等指标 ,同时还对各组心肌细胞超微结构进行了电镜观察。结果 :　以上各组 GOT除安静对照组与相应沙棘组和训练即刻组与训练恢复组间无显著差异外 ( P>0 .0 5 ) ,其余各组间均有显著差异 ( P<0 .0 5 ) ;SOD除安静对照组与相应沙棘组 ,训练即刻沙棘组与训练恢复沙棘组间无显著差异外 ( P>0 .0 5 ) ,其余各组间均有显著差异 ( P<0 .0 5 ) ;MDA除训练即刻组与相应沙棘组及恢复组间无显著差异外 ( P>0 .0 5 ) ,其余各组间均有显著差异 ( P<0 .0 5 ) ;沙棘组心肌细胞中线粒体、肌浆网、肌丝等结构均比相应对照组完整 ,病变程度比对照组轻。结论 :　沙棘油能改善运动对心肌细胞的损伤 ,提高心肌组织抗自由基氧化的能力 ,同时还对心肌细胞超微结构具有明显保护作用。

EFFECT OF HIPPOPHAE RHAMNOIDES OIL ON METABOLISM OF FREE RADICALS AND ULTRASTRUCTURE OF MYOCARDIUM IN MICE

Objective: The effect of oil of Hippophae rhamnoides (OHR) supplements on sGOT,metabolism of free radical and ultrastructure of myocardium was observed in mice.Methods: 70 male ICR mice were randomly divided into six groups: sedentary control group (SCG), sedentary+OHR group (SHG), immediately after exhaustive exercise in control group (IEC) and in OHR group (IEO), 24 hours recovery after exhaustive exercise in control group (REC) and in OHR group (REO). After 6 w swimming program with gradually increasing intensity, these mice carried out an exhaustive swimming before sacrifice. Serum GOT, SOD and MDA in myocardium were measured and the changes of ultrastructure of myocardium were observed by transmission electron microscope (TEM). Results: 1) Serum GOT was significantly different among these groups except between SCG and SHG, IEC and REC. 2) SOD was significantly different among the above groups except between SCG and SHG, IEO and REO. 3) The concentration of MDA was significantly different among the above groups except between IEC and IEO, REC and REO. 4) The integrity of mitochondrion, rough surfaced endoplasmic reticulum and myofilament in myocardium was better in OHR groups than in control groups and the pathological tendency of these ultrastructures was lighter in OHR groups compared with control groups. Conclusion: OHR supplementation can reduce the damage of myocardium caused by exercise and enhance antioxidative status of myocardium; OHR supplementation can protect the ultrastructure of myocardium.