复健片对MCAO大鼠脑组织GFAP表达及局部脑血流量的影响

目的:探讨复健片对MCAO模型大鼠星形细胞活性及其局部脑血流灌注状态的影响。方法:选取健康雄性Wistar大鼠,随机分为3组,即假手术组、模型对照组(简称模型组)、药物组各24只。采用右侧近端大脑中动脉电凝术,制作大脑中动脉闭塞(middle cerebral artery occlusion,MCAO)模型。按要求分别给予复健片水溶液、生理盐水灌胃2周。采用激光多普勒血流仪测定梗死侧额叶局部脑血流量(regional cerebral blood flow,rCBF);利用免疫组织化学方法,观察复健片对MCAO大鼠脑组织胶质纤维酸性蛋白(glial fibrillary acid protein,GFAP)表达的影响。指标间的相关性采用直线相关分析。结果:rCBF测量结果表明,术后模型组与药物组梗死侧额叶皮质rCBF均显著低于假手术组(P<0.01);而经药物治疗1周后,梗死侧额叶皮质的rCBF虽然仍低于假手术组水平(P<0.05),但较之治疗前和同期模型组,均有明显增加(P<0.05);经药物治疗2周后,梗死侧额叶皮质的rCBF仍高于同期模型组(P<0.05);模型组与假手术组在观察的各时点则均无显著性变化。免疫组化结果显示,大脑中动脉阻塞后,模型大鼠梗死灶周围区GFAP的表达同假手术组相比并无显著改变(P>0.05);而药物组分别给药1周、2周后,GFAP的表达均较同期模型组、假手术组及药物组术后1周有显著增强(P<0.05)。药物灌胃1周、2周组的GFAP平均灰度值和rCBF相关分析表明,两者具有明显相关性。结论:复健片可以增强MCAO模型大鼠脑组织星形胶质细胞的活性,而且这种活性的上调有助于改善受损脑组织的局部血流量。

Effects of Fujian Tablets on the Expression of GFAP and rCBF in the Brains of MCAO Model Rats

Objective:To investigate the correlation of the changes of the expression of GFAP with rCBF in the brains of MCAO rat models affected by Fujian tablets.Methods:72 healthy male Wistar rats were randomly divided into three groups including sham operation group,model group and administration group.Middle cerebral artery occlusion models were established by electrocoagulation.The solution was Fujian tablets was administrated in administration group and equal volume of normal saline was administrated in sham operation group(P<0.01)and model group by gavage for 2 weeks respectively.Laser Doppler Flowmetry(LDF)was used to measure rCBF in lesioned frontal lobe cortex.Immunohistochemistry was used to observe the expression of GFAP in the lesioned cortex.Linear correlation analysis was used to analyze the correlation of changes of the expression of GFAP with those of rCBF.Results:After operation,rCBF in the lesioned frontal lobe cortex of the rats of model group and administration group both decreased remarkably compared with sham operation group(P<0.05).But after administration of Fujian tablets for 1 week,rCBF in the lesioned frontal lobe cortex increased remarkably(P<0.05,vs before administration)and is also more than in model group in synchronization(P<0.05),though it is still lower than that in sham operation group.After administration for 2 week,rCBF is still more than that in model group in synchronization.In model group little changes occurred in rCBF as well as in sham operation group.Immunohistochemistry showed after operation little difference in the expression of GFAP exists between these three groups(P>0.05).After administration of Fujian tablets for 1 week and 2 weeks,both the expressions of GFAP enhanced compared with other two groups in synchronization and administration group 1 week after operation(P<0.05).Linear correlation analysis demonstrated rCBF is closely related to the expression of GFAP in the administration group after administration for 1 week and 2 weeks.Conclusion:Fujian tablets can improve the activity of astrocytes in brains of model rats,which can facilitate increasing rCBF in lesioned cortex.