分化抗原簇CD38在慢性淋巴细胞白血病T淋巴细胞中的表达研究

目的 探讨慢性淋巴细胞白血病(CLL)中T淋巴细胞CD38(CD38-T)的表达特征.方法 应用多参数流式细胞术检测83例CLL患者肿瘤细胞中CD38表达(CD38-B)、zeta链相关蛋白-70(ZAP-70)以及CD38-T、CD4/CD8比值的表达情况.结果 在所有患者中,CD38+-T占49.4%(41/83),CD38+-B占50.6%(42/83),CD38+-T和CD38-B表达有高度相关性(r=0.553,P<0.01);表达ZAP-70+CD38+-T和ZAP-70-CD38+-T的患者占67.5%(56/83),CD38-T与ZAP-70的表达有相关性(r=0.349,P<0.01).Binet A期患者中CD38+-T占33.3%(14/42),Binet B+C期患者中CD38+T占65.9%(27/41);XD38+-T与临床分期,CD4/CD8比例倒置有相关性(r=0.312、0.453,P<0.05).结论 CD38-T表达变化与患者病情进展及转归密切相关,CD38高表达的患者多伴有免疫功能调节紊乱或失衡,可作为CLL的一项全新独立的监测指标.

Expression of CD38 in T cells of chronic lymphocytic leukemia

Objective To explore the CD38 expression in T-cens(CD38-T)of chronic lymphocytic leukemia(CLL).Methods Multi-parameter flow cytometry was used to detect the expression of CD38,ZAP-70 and CD4/CD8 in 83 CLL patients.Results CD38 was positively expressed in 49.4%(41/83)T-cells(CD38-T)and 50.6%(42/83)tumor cells(CD38-B)of all CLL patients.The expression of the CD38-T was highly correlated with CD38-B(r=0.553,P＜0.01).ZAP-70+CD38+-T and ZAP-70-CD38-T constituted 67.5%(56/83)of CLL patients.There was significant correlation between CD38-T and ZAP-70 (r=0.349,P＜0.01).There was 33.3%(14/42)patients with CD38-T in Binet A patients,and 65.9% (27/41)in Binet B+C patients.There was also significant correlation between CD38-T and Binet B+C(r=0.312,P＜0.05),and CD4/CD8(r=0.453,P＜0.05).Conclusions Aberrant expression of CD38 in T-cells might be of prognostic relevance in CLL Most of the patients with high level CD38-T expression are accompanied with disorder or imbalance of immune function.CD38 expression in T cells might be a new prognostic factor in CLL.