| 重组人肝再生增强因子可抑制大鼠肝星状细胞c-fos基因的表达 |
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| 引用本文： | 王爱民,向英,耿焱,杨晓明,马志杰,楚瑞珏,王宝恩. 重组人肝再生增强因子可抑制大鼠肝星状细胞c-fos基因的表达[J]. 中华实验外科杂志, 2001, 26(1): 900-901. DOI: 10.3760/cma.j.issn.1001-9030.2009.07.031 |
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| 作者姓名： | 王爱民 向英 耿焱 杨晓明 马志杰 楚瑞珏 王宝恩 |
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| 作者单位： | 解放军252医院消化科,河北保定,071000;军事医学科学院放射医学研究所;北京友谊医院肝病中心; |
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| 基金项目： | 军队杰出人才项目军队面上A类基金 |
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| 摘 要： | Objective To investigate the effects of recombinant human augmenter of liver regen-eration (hALR) on c-fos gene expression in hepatic stellate cells (HSCs). Methods HSC-T6 cells were cultured in medium containing different concentrations of hALR (200,20,2 μg,/L respectively), and col-lected at different incubation periods (8,24,48,72 h). Total RNA of HSCs was isolated, and the c-fos gene expressions levels were detected by reverse transcription polymerase chain reaction. Results The C-fos gene expression levels of HSCs in high (200 μg/L), middle (20 μg/L) and low (2 μg/L) concentra-tions of hALR groups were significantly lower than those in control group after exposure to hALR for 8,24, 48 and 72 h. The expression of C-fos gene in low,middle,and high hALR groups was 50%-53% ,38%-43% ,and 26%-30% of control group,respectively. Conclusion hALR could inhibit the gene expression of c-los in HSCs.
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| 关 键 词： | 肝星状细胞 基因表达 |
Inhibitory effects of recombinant human augmenter of liver regeneration on gene expression of c-fos in hepatic stellate cells of rats |
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| Abstract: | |
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| Keywords: | c-fasHepatic stellate cellc-fosGene expression |
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