谷丙转氨酶GPT2对急性髓系白血病细胞HL60生长特性的影响

目的:探讨参与三羧酸(TCA)循环中谷氨酰胺代谢的谷丙转氨酶2(glutamic pyruvate transaminase 2,GPT2)对急性髓系白血病细胞生长、增殖及凋亡的影响.方法:数据库挖掘了解不同血液肿瘤以急性白血病细胞中GPT2的表达水平,通过构建目的基因short-hairpin RNA (shRNA)...

The Influence of Glutamic Pyruvate Transaminase 2 to Biological Characteristics of Acute Myeloid Leukemia Cell HL60

Objective:To investigate the influence of GPT2(glutamic pyruvate transaminase 2)to biological characteristics of human acute myeloid leukemia cell line HL60.Methods:The expression of GPT2 in hematological tumor and AML cell was detected.The lentvirus-mediated of short-hairpin RNA(shRNA)was constricted,and the knock-down efficiency of HL60 in AML cell after infected by lentvirus-mediated was detected by Western blot and QPCR.CCK-8 assay and soft agar colony formation assay were used to detect the effect of GPT2 gene deletion to the cell proliferation potential.Fluorescence activated cell sorting(FACS)was used to analyze the effect of gene deletion to the cell cycle and Caspase 3/7 Activity Assay Kit was used to analyze the effect of GPT2 gene deletion to the cell apoptosis.Results:GPT2 showed mRNA high expression in AML patients.CCK-8,soft agar assay,and Caspase 3/7 Activity Assay Kit results showed that compared with shCtrl group,the cells in shGPT2-1、shGPT2-2、shGPT2-3 group showed the slowing down on proliferation,decreasing on colony ability,and the apoptosis of the cells was increasing significantly.FACS showed that GPT2 gene was related to the cycle of HL60 cell.Conclusion:GPT2 appears to involve the proliferation,cycle distribution and apoptosis of AML cell HL60.The deletion of GPT gene can lead to the inhibitation of cells proliferation and increase apoptosis.