广泛性焦虑障碍与5-羟色胺转运体基因多态性的相关研究

目的　探讨广泛性焦虑障碍与 5 羟色胺转运体 (5 HTT)基因启动子区和内含子 2区两种多态性的相关性。方法　运用聚合酶链反应技术检测 4 7例广泛性焦虑障碍患者 (患者组 )和 90名健康对照者 (对照组 )两种基因多态性的分布频率。结果　患者组启动子区多态性 (5 HTTLPR)的short/short(SS)基因型和short(S)等位基因频率分别为 72 %和 83% ,对照组SS基因型和S等位基因频率分别为 4 9%和 71% ,两组间的差异有显著性 (P <0 0 5 )。内含子 2区数目可变的顺向重复多态性各基因型 (12 / 12 ,12 / 10 ,10 / 10 )频率在患者组中分别为 72 % ,2 6 % ,2 % ,在对照组中分别为 78% ,2 1% ,1% ,两组间的差异无显著性 (P >0 0 5 ) ;等位基因频率比较的差异亦无显著性 (P >0 0 5 )。结论　 5 HTTLPR的SS基因型可能是广泛性焦虑障碍的易感基因之一。

Association study of serotonin transporter gene polymorphisms and generalized anxiety disorder

Objective To explore the association between generalized anxiety disorder (GAD) and polymorphisms of serotonin transporter (5-HTT). Methods Using a polymerase chain reaction-based technique, polymorphisms of two regions, 5-HTT-linked polymorphic region (5-HTTLPR) and VNTR in intron 2, in serotonin transporter gene were determined in 47 patients with GAD and 90 control subjects, the allelic and genotypic distributions in two groups were compared. Cases and controls were matched for ethnic and geographic origin. Results The frequencies of 5-HTT short/short (SS) genotype and allele S were significantly higher in GAD patients than in controls (72% vs. 49%; 83% vs. 71%; both P< 0.05). However, there was no difference on the distribution of VNTR in intron 2 (12/12, 12/10, 10/10) between patients (72%,26%,2%) and controls (78%,21%,1%). Conclusion The findings support the presence of 5-HTTLPR allele S may increase the risk for generalized anxiety disorder.