| 川芎嗪对血小板中肌醇磷脂和2OK蛋白质磷酸化的抑制作用 |
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| 引用本文： | 丁克宏,梁念慈.川芎嗪对血小板中肌醇磷脂和2OK蛋白质磷酸化的抑制作用[J].中国药理学通报,1992(5). |
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| 作者姓名： | 丁克宏 梁念慈 |
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| 作者单位： | 湛江医学院生化教研室，湛江医学院生化教研室 湛江524023 衡阳医学院生化研究室 421001，湛江524023 |
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| 基金项目： | 国家自然科学基金资助课题 |
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| 摘 要： | 在Mg~(2+)存在下.用血小板膜与(γ—~(32)P)ATP保温,以观察川芎嗪对~(32)P掺入磷脂和蛋白质的影响,结果表明:血小板膜中存在磷脂酰肌醇(PI)激酶和磷脂酰肌醇—4—磷酸(PIP)激酶,而胞浆中缺乏或极少;ATP促进肌醇磷脂磷酸化;川芎嗪抑制血小板中PIP和20K蛋白质的磷酸化.半数抑制浓度分别为40μmol·L~(-1)和110μmol·L
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| 关 键 词： | 川芎嗪 血小板 磷脂酰基 肌醇类 磷酰化作用 |
Inhibition of tetramethylpyrazine on the phosphorylation of phosphoinositide and 20K protein in platelets |
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| DING Ke-Hong. LIANG Nian-Ci.Inhibition of tetramethylpyrazine on the phosphorylation of phosphoinositide and 20K protein in platelets[J].Chinese Pharmacological Bulletin,1992(5). |
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| Authors: | DING Ke-Hong LIANG Nian-Ci |
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| Abstract: | Thrombocytic membranes were incubated with μ-32P] ATP in the presence of Mg2+ to observe the effect of tetramethylpyrazine on incorporation of 32 P into phospholipids and proteins. The result showed there are phosphatidylinositol kinase and phosphatidylinositol 4 - phosphate kinase in platelet membranes, while almost none or very little in cytosol. ATP stimulated the phosphory-lation of phosphoinositides. Tetram-
ethylpyrazine inhibited the phosphoryla-tion of phosphatidylinositol 4-phosphate and 20K protein in platelets. Concentrations giving 50% inhibition (IC50) were 40 μmol · L-1 and 110 μmol · L-1 respectively. The data might be valuable in clarifying the antiplatelt mechanism of tetramethylpyrazine. |
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| Keywords: | tetramethylpyrazine blood phatelets phosphatidylinositols phosphorylation |
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