Preparation and in vitro evaluation of bFGF-loaded chitosan nanoparticles |
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| Authors: | Cetin Meltem Aktas Yesim Vural Imran Capan Yilmaz Dogan Lale A Duman Memed Dalkara Turgay |
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| Affiliation: | a Department of Pharmaceutical Technology, Faculty of Pharmacy, Hacettepe University, Ankara, Turkeyb Department of Basic Oncology, Faculty of Medicine, Institute of Oncology, Hacettepe University, Ankara, Turkeyc Department of Chemical Engineering and Bioengineering Division, Hacettepe University, Ankara, Turkeyd Department of Neurology, Faculty of Medicine, Hacettepe University, Ankara, Turkey |
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| Abstract: | ![]()
The objective of our study was to prepare and characterize basic fibroblast growth factor (bFGF)-loaded nanoparticles. Protein-loaded chitosan nanoparticles were obtained by ionotropic gelation process based on the interaction between chitosan and tripolyphosphate (TPP). The protein-loading capacity and encapsulation efficiency were 0.021% and 27.388%, respectively. The bFGF-loaded nanoparticles have a mean diameter of 424 nm, a narrow size distribution, spherical shape and positive surface charges. In vitro release showed that the extent of release was 68% at 24 hr. The protein integrity was investigated by SDS-PAGE analysis that confirmed protein integrity was not affected by the encapsulation procedure and release conditions. |
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| Keywords: | bFGF Chitosan Nanoparticles SDS-PAGE TPP |
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