胃炎饮对实验性胆汁反流性胃炎大鼠胃黏膜COX-2mRNA表达的影响

目的 探讨胃炎饮对实验性胆汁反流性胃炎大鼠胃黏膜环氧合酶-2(COX-2)mRNA表达的影响.方法 采用自制反流液灌胃建立实验性胆汁反流性胃炎大鼠模型,以吗叮啉为对照,采用RT-PCR技术观察胃炎饮对实验性胆汁反流性胃炎大鼠胃黏膜COX-2mRNA表达以及病理组织学的影响.结果 模型组大鼠胃黏膜出现明显破损、脱落,黏膜下可见大量炎细胞浸润,大部分大鼠可见不典型增生和肠上皮化生,同时胃黏膜COX-2mRNA表达升高(P<0.01),与模型组比较,胃炎饮可明显改善实验性胆汁反流性胃炎大鼠胃黏膜病理改变,并降低COX-2mRNA表达(P<0.05或P<0.01).结论 胃炎饮具有降低模型大鼠胃黏膜COX-2mRNA表达,防止胃黏膜肠上皮化生、不典型增生等癌前病变发生的作用.

Effect of Weiyanyin on the expression of Cyclooxygenase-2mRNA in gastric mucosa of rats with experimental bile reflux gastritis

Objective To observe the effect of Weiyanyin on the expression of Cyclooxygenase-2(COX-2)mRNA gastric mucosa of rats with experimental bile reflux gastritis.Methods The model of experimental bile reflux gastritis was eshtablished by feeding reflux liquid.Domperidone was taken as control.The effect of Weiyanyin on pathological histology was observed and the expression of COX-2mRNA in gastric mucosa was detected by method of RT-PCR.Results The gastric mucosa of rats in the model group was damaged,amotic,and a lot of inflammatory cells could be seen.Most of rats showed glandular organ hyperplasy and intestinal metaplasia.At the same time the expression of COX-2mRNA were increased(P<0.01).Compared with the model group,Weiyanyin could improve pathological histology of experimental bile reflux gastritis rats,and decrease the expression of COX-2mRNA(P<0.05 or P<0.01).Conclusion Weiyanyin could decrease the expression of COX-2mRNA to prevent mucosal precancerous change such as Intestinal metaplasia,atypical hyperplasia and so on.