乙酸溶解-酶消化法制备医用胶原膜

目的:探讨I型胶原的提取和胶原膜的制备方法。方法：取新鲜成年牛跟腱，用乙酸溶解-酶消化法提取I型胶原，并进行盐析、透析、纯化得到精制的液态胶原，用戊二醛进行交联。结果：提取的胶原紫外分光光谱最高吸收峰值为230 nm左右；聚丙酰胺凝胶电泳呈现2条相邻带(相对分子质量98 000左右)，符合I型胶原的特性。冻干交联胶原膜呈白色海绵状，具有一定弹性。扫描电镜观察胶原膜为孔网状结构，孔径15～50 μm。结论：乙酸溶解-酶消化法提取牛腱胶原是一种简单、理想的方法，可得到含量及纯度较高的Ι型胶原。

Preparation of medical collagen membrane with acetic acid dissolution and enzyme digestion

Objective To investigate the extraction of type I collagen and the preparation of collagen membrane. Methods Fresh adult bovine tendon was dissolved with acetic acid and enzyme-digestion for type I collagen. Then they were dialysed and purified for refined liquid collagen, which was cross-linked with glutaraldehyde.Results The maximum absorbance of the purified collagen appeared at 230 nm， with correspondence to type I collagen，two close bands on SDS-PAGE (mol.wt 98 000) suggested the purified product was type I collagen. The extracted collagen was white, with no smell. The collagen membrane was white, sponge-like and elastic. SEM showed structure of holes and nets of collagen membrane, the diameter of which was 15-50 μm. Conclusion:The method of acetic acid dissolution and enzyme digestion is a simple and ideal one for extracting bovine tendon collagen. The type I collagen is much and highly purified with low antigen.