葛根素对大鼠心肌缺血再灌注损伤的保护作用及抗氧化应激机制的探讨

目的：探讨葛根素对大鼠心肌缺血再灌注损伤的保护作用（Myocardial Ischemia-reperfusion Injury,MIRI）及抗氧化应激机制。方法：大鼠心肌缺血30min后再灌注180min造成大鼠心肌缺血再灌注损伤模型（灌注24 h,用于测定心肌梗死面积）。实验组和对照组分别给予葛根素和生理盐水。收集大鼠心脏,观察大鼠心肌缺血区的心肌细胞凋亡情况;收集血清测定其抗氧化应激的指标。结果：与模型组相比,葛根素剂量依赖性的减少了MIRI大鼠的心肌细胞凋亡、心梗面积和血清中丙二醛的含量,增加了血清中超氧化物歧化酶、谷胱甘肽过氧化物酶的活性和谷胱甘肽的含量。结论：葛根素对MIRI大鼠具有抗氧化应激的作用,它能够剂量依赖性的减少心肌细胞凋亡,最终减少心肌梗死面积。

Puerarin Protects Heart Against Oxidative Stress Induced by Acute Myocardial Ischemia-Reperfusion Injury in Rats

Objective：To investigate the protective mechanism of puerarin on acute Myocardial Ischemia-reperfusion Injury（MIRI）rats,and to explain the antioxidative mechanism involved.Methods：MIRI rat model was induced by ischemia for 30 min and then reperfusion for 180 min.At the end of the 3h reperfusion period（or 24h for infarct size）,we measured the myocardial infarct size,myocardial apoptosis and the activities of antioxidative enzymes.Results：Puerarin reduced infarct size,myocardial apoptosis and the serum level of malondialdehyde,increased the activities of superoxide dismutase and glutathione-peroxidase activities and serum level of glutathione.Conclusion：Puerarin exerts significant cardioprotective effects against MIRI in rats,likely through its antioxidation and antilipid peroxidative properties.