恶性肿瘤患者外周血CD4+CD25high Foxp3+调节性T细胞的检测及临床意义

目的：研究CD4⁺CD25^high Foxp3⁺调节性T细胞(Tregs)在恶性肿瘤患者外周血单个核细胞(PBMC)中的表达,探讨其在肿瘤发病中的作用及临床意义。方法：采用流式细胞术检测61例不同分期恶性肿瘤患者PBMC中CD4⁺CD25⁺/CD4⁺CD4⁺CD25^high/CD4⁺T细胞百分比，进一步分析CD4⁺CD25⁺及CD4⁺CD25^highT细胞中表达Foxp3⁺T细胞的百分比，并与正常对照组(n=32)进行比较。结果：与正常对照组比较，Ⅰ及Ⅱ期恶性肿瘤患者PBMC中CD4⁺CD25⁺/CD4⁺及CD4⁺CD25^high/CD4⁺T细胞百分比均无明显变化(P>0.05),Ⅲ及Ⅳ期恶性肿瘤患者均明显增高(P<0.05)，随肿瘤恶性程度增高，CD4⁺CD25⁺/CD4⁺及CD4⁺CD25^high/CD4⁺T细胞的百分比也逐渐增高；恶性肿瘤患者CD4⁺CD25⁺ 及CD4⁺CD25^high Foxp3⁺T细胞表达水平较正常对照组均增高,组间比较差异均有显著性(P< 0.01)。结论:恶性肿瘤患者外周血调节性T细胞增高可能是恶性肿瘤发病及免疫机制异常的主要原因之一，定期检测外周血中上述指标的变化，有利于了解恶性肿瘤患者的病情变化及预后判断。

Measurement of CD4+CD25high Foxp3+ regulatory T cells in peripheral blood of patients with cancer and clinical significance

Objective To study the expression of CD4+ CD25high Foxp3+ regulatory T cells (Tregs) in peripheral blood monouclear cell(PBMC) of patients with cancer,and explore the significance of Tregs in pathogenesis of cancer.Methods The proportions of CD4+ CD25+/ CD4+,CD4+ CD25high / CD4+T cells in PBMC were examined with flow cytometry in 61 patients with cancer at different stages,and the expression levels of Foxp3+ in CD4+ CD25+ and CD4+ CD25high T cells were analyzed,and compared with 32 normal control samples.Results Compared with normal controls,the proportions of CD4+ CD25+/ CD4+,CD4+ CD25high / CD4+T cells in PBMC of patients with cancer at stageⅠand stage Ⅱ had no significant difference(P>0.05);but in those patients at stage Ⅲ and stage Ⅳ,they were higher than those in normal controls,the difference was significant(P<0.05).The proportions of CD4+CD25+/ CD4+,CD4+ CD25high / CD4+T cells were higher in PBMC of patients with cancer with higher histophathological grade.The expression levels of Foxp3+ in CD4+ CD25+ and CD4+ CD25high T cells in patients with cancer were also higher than those in normal controls(P<0.01),the differences were significant(P<0.01).Conclusion The increase of Tregs in patients with cancer may be related to tumor progression and it shows immunological abnormality in patients with cancer.The regular detection of CD4+ CD25+,CD4+ CD25high and CD4+ CD25high Foxp3+ in PBMC will contribute to understanding the patient’s condition and judging prognosis.