MMP-2与TIMP-1在食管鳞癌细胞中的表达及临床意义

目的：检测食管鳞癌（ESCC）及癌旁正常组织中基质金属蛋白酶-2（MMP-2）以及组织抑制因子（TIMP-1）的表达，研究其与临床特征的关系，为临床ESCC恶性程度和预后判断提供新的依据。方法应用组织芯片技术及免疫组织化学链霉菌抗生物素蛋白-过氧化酶连接法（S-P法）对60例ESCC组织及癌旁正常食管组织中MMP-2、TIMP-1蛋白表达进行检测，分析其阳性表达与性别、年龄、组织学分级、浸润深度、淋巴结转移及肿瘤分期之间的关系。结果 MMP-2、TIMP-1在癌旁正常上皮组织不表达；MMP-2主要在癌细胞中表达， TIMP-1主要在间质细胞中表达，二者在食管鳞状细胞癌中表达阳性率分别为38.3%和46.7%，高于正常组织（P﹤0.05）。MMP-2与ESCC的浸润深度、淋巴结转移和TNM临床分期有关，差异有统计学意义（P﹤0.05），肿瘤浸润程度深者MMP-2阳性表达率高；无淋巴结转移者的MMP-2阳性表达率低于有淋巴结转移者；临床病理分期为Ⅰ、Ⅱ期患者的阳性表达率低于Ⅲ期者。TIMP-1的表达与ESCC性别、年龄、组织学分级、浸润深度、淋巴结转移及临床病理分期无关（P﹥0.05）。结论 MMP-2能在ESCC的侵袭、转移中起重要作用，TIMP-1可能与早期ES-CC的生物学性状有关，MMP-2与浸润深度、淋巴结转移及TNM分期有关，二者有助于判断ESCC的生物学行为。

Expressions and clinical significance of MMP-2 and TIMP-1 in esophageal squamous cell carcinoma

Objective To investigate the association between the expressions of matrix metallproteinase-2 (MMP-2), the tissue inhibitor of matrix metallproteinase-1 (TIMP-1) and the biological characters of esophageal squamous cell carci-noma (ESCC). Method The expressions of MMP-2 and TIMP-1 were determined by immunohistochemical analysis (S-P method) with tissue chip technique in 60 cases of ESCC. Statistical analyses were performed to determine the associa-tion between positive expressions of MMP-2, TIMP-1 and gender, age, histological stage, tumor invasion and metastasis. Result The expressions of MMP-2 and TIMP-1 were not detected in adjacent normal epithelial tissues, while MMP-2 expression was predominantly observed in cancer cells, and TIMP-1 expression was mainly in cytoplasm, with a positive expression rate of 38.3% and 46.7% in ESCC, respectively, both were higher as compared in normal tissues (P<0.05). The expression of MMP-2 were correlated with the depth of cancer invasion, lymph node metastasis, and TNM stage (all P<0.05), patients with deeper invasion, or with metastasis, or with stage III rather than stage I and II had higher expres-sion of MMP-2. While expression of TIMP-1 was not related to gender, age, histological stage, invasion, lymph node me-tastasis and clinicopathological stage (P>0.05). Conclusion The high expression of MMP-2 may be essential in ESCC invasion and metastasis, while the TIMP-1 may be associated with early ESCC biological characteristics, the expression of MMP-2 was correlated with invasion depth, lymph node metastasis and TNM stage, which will be helpful in predicting the biological behaviors of ESCC.