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保健品中芍药苷含量的二极管阵列反相高效液相色谱测定法
引用本文:孙艳芳,陈彦凤,刘长福,刘秀梅,付宇. 保健品中芍药苷含量的二极管阵列反相高效液相色谱测定法[J]. 职业与健康, 2012, 28(19): 2358-2359
作者姓名:孙艳芳  陈彦凤  刘长福  刘秀梅  付宇
作者单位:黑龙江省疾病控制中心,哈尔滨市,150030
摘    要:目的建立用反相高效液相色谱测定保健食品、保健用品中功效成分芍药苷的方法。方法样品中芍药苷经甲醇或水提取、C18反相柱分离,在波长232 nm处检测。结果方法的回收率为96.3%~100.4%,RSD为1.4%~1.7%。结论该方法简便快速,准确可靠,可用于各类保健品中芍药苷含量的测定。

关 键 词:芍药苷  液相色谱法  功效成分

Determination of paeoniflorin in health products by reversed phase high performance liquid chromatography with diode array
SUN Yan-fang , CHEN Yan-feng , LIU Chang-fu , LIU Xiu-mei , FU Yu. Determination of paeoniflorin in health products by reversed phase high performance liquid chromatography with diode array[J]. Occupation and Health, 2012, 28(19): 2358-2359
Authors:SUN Yan-fang    CHEN Yan-feng    LIU Chang-fu    LIU Xiu-mei    FU Yu
Affiliation:Heilongjiang Center for Disease Control and Prevention,Harbin,150030,China
Abstract:[Objective]To establish a method for determination of paeoniflorin in health foods and health supplies by reversed phase high performance liquid chromatography.[Methods]Paeoniflorin in samples were extracted by methanol or water,separated by C18 reversed phase column,and detected at a wavelength of 232 nm.[Results]The recovery of this method was 96.3%-100.4%,and RSD was 1.4%-1.7%.[Conclusion]The method is simple,rapid,accurate and reliable,which can be used for the determination of paeoniflorin in health products.
Keywords:Paeoniflorin  Liquid chromatography  Functional component
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