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| 巴豆总生物碱的纯化工艺研究 | |
| 引用本文： | 肖祖平,曾宝,李生梅,唐君苹,林吉,赖小平. 巴豆总生物碱的纯化工艺研究[J]. 中南药学, 2012, 10(10): 737-739 |
| 作者姓名： | 肖祖平 曾宝 李生梅 唐君苹 林吉 赖小平 |
| 作者单位： | 1. 广州中医药大学,广州,510006 2. 广州中医药大学,广州510006;东莞广州中医药大学中医药数理工程研究院,广东东莞523808 |
| 基金项目： | 国家科技部重大新药创制专项(编号:2009ZX09103-388);广东省科技计划项目(编号:2009A030100014) |
| 摘 要： | 目的 研究大孔树脂分离纯化巴豆总生物碱的工艺.方法 采用分光光度法测定总生物碱,筛选HPD400、AB-8、NKA-9、ADS-17、D101树脂对巴豆总生物碱的吸附和解吸附能力,并优化分离纯化条件.结果 优选出D101树脂,上样液浓度为生药1.5 g ·mL-1,吸附流速1.5 BV·h-1,药材量∶树脂量=9∶2,4 BV水除杂后用4 BV 30％乙醇冲洗收集目标滤液.结论 该方法简便易行,适合于巴豆总生物碱的分离纯化. |
| 关 键 词： | 巴豆 总生物碱 大孔树脂 纯化 |
Purif ication of total alkaloids from Croton tiglium L. |
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| XIAO Zu-ping , ZENG Bao , LI Sheng-mei , TANG Jun-ping , LIN Ji , LAI Xiao-ping. Purif ication of total alkaloids from Croton tiglium L.[J]. Central South Pharmacy, 2012, 10(10): 737-739 | |
| Authors: | XIAO Zu-ping ZENG Bao LI Sheng-mei TANG Jun-ping LIN Ji LAI Xiao-ping |
| Affiliation: | 1,2(1.Guangzhou University of Chinese Medicine,Guangzhou 510006;2.Research Institute of Mathematical Engineering,Guangzhou University of Chinese Medicine,Dongguan Guangdong 523808) |
| Abstract: | Objective To determine the technology for purif ication of total alkaloids from Croton tiglium L.by macroporous resin.Methods Acidic dye colorimetry was used to determine the total alkaloids.The adsorption and desorption performances of HPD400,AB-8,NKA-9,ADS-17 and D101 macroporous resins were compared and purification conditions optimized.Results D101 resin was selected because it was separated most effectively.The concentration of sample solution was 1.5 g · mL-1.The adsorption flow rate was 1.5 BV · h-1.Crude medicinal material to resin was 9 : 2,then 4 BV water and 4 BV 30% ethanol were used as the eluting solvent to wash the resin,collecting 30% ethanol elution.Conclusion This method is simple and feasible,which can be used to separate and purify total alkaloids from Croton tiglium L.. |
| Keywords: | Croton tiglium L. total alkaloids macroporous resin purif ication |
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