地塞米松预处理人乳腺癌MCF-7细胞对多西他赛抗肿瘤活性的影响

目的 探讨地塞米松预处理人乳腺癌MCF-7细胞后,对化疗药物多西他赛(艾素)抗肿瘤活性的影响.方法 以不同浓度(1×10-8～1×10-6 mol·L-1)的地塞米松预先作用于人乳腺癌MCF-7细胞后,再以艾素(5×10-6 mol·L-1)处理,在以上药物作用的相应时间段,通过流式细胞仪技术测定细胞凋亡,采用细胞计数观察细胞生长密度、形态.结果 地塞米松对MCF-7细胞增殖有抑制作用(P＜0.01),艾素能够明显抑制MCF-7细胞增殖,促进其凋亡(P＜0.01),地塞米松预处理后艾素干预MCF-7细胞的增殖抑制作用较单用艾素减弱(P＜0.01),且随地塞米松浓度的增加,其对化疗药艾素的凋亡抑制作用的影响增强.结论 地塞米松预处理对艾素诱导的人乳腺癌MCF-7细胞的凋亡具有明显的阻抑作用,抑制了多西他赛的抗肿瘤活性.

Anti-tumor activity of docetaxel in human breast cancer cell line MCF-7 pretreated by dexamethasone

Objective To investigate the antineoplastic activity of docetaxel in human breast cancer cell line MCF-7 pretreated by dexamethasone(Dex).Methods With docetaxel contributed to the MCF-7 cells pretreated with dexamethasone at diverse concentration,flow cytometer was utilized to detect the cell apoptosis subsequently.The cells were counted and observed.Results Either Dex or docetaxel suppressed the generation of MCF-7 cells(P<0.01).The combined effect of the two drugs was attenuated,and the suppressive effect on the generation of docetaxel was lowered after Dex pretreatment(P<0.01).Conclusion The antineoplastic activity of docetaxel in human breast cancer cell line MCF-7 can be suppressed by dexamethasone.