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| 蒽酮硫酸法与苯酚硫酸法测定竹节参多糖含量的比较研究 | |
| 引用本文： | 张杰,李春艳,李劲平,潘娟,向大雄. 蒽酮硫酸法与苯酚硫酸法测定竹节参多糖含量的比较研究[J]. 中南药学, 2012, 10(6): 421-424 |
| 作者姓名： | 张杰 李春艳 李劲平 潘娟 向大雄 |
| 作者单位： | 1. 中南大学湘雅三医院,长沙410013;中南大学药学院,长沙 410013 2. 中南大学药学院,长沙,410013 3. 中南大学湘雅二医院,长沙,410011 |
| 基金项目： | 湖南省中医药科研基金计划项目(编号:2009062) |
| 摘 要： | 目的 考察蒽酮硫酸法与苯酚硫酸法测定竹节参多糖(Panax japonicus polysaccharides,PJPS)含量的可行性并比较其差异,建立竹节参多糖含量测定方法.方法 对蒽酮硫酸法和苯酚硫酸法测定竹节参多糖含量的显色条件和方法学进行研究,比较2种方法测定结果的差异.结果 蒽酮硫酸法含量测定结果高于苯酚硫酸法,其差异有统计学意义,蒽酮硫酸法的线性、重现性和稳定性都优于苯酚硫酸法.结论 蒽酮硫酸法比苯酚硫酸法更适合测定竹节参中多糖的含量. |
| 关 键 词： | 竹节参 多糖 蒽酮硫酸法 苯酚硫酸法 含量测定 |
Determination of polysaccharide in rhizoma of Panax japonicus by anthrone sulfuric acid method and phenol sulfuric method |
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| ZHANG Jie , LI Chun-yan , LI Jin-ping , PAN Juan , XIANG Da-xiong. Determination of polysaccharide in rhizoma of Panax japonicus by anthrone sulfuric acid method and phenol sulfuric method[J]. Central South Pharmacy, 2012, 10(6): 421-424 | |
| Authors: | ZHANG Jie LI Chun-yan LI Jin-ping PAN Juan XIANG Da-xiong |
| Affiliation: | 1.Third Xiangya Hospital,Central South University,Changsha 410013;2.School of Pharmaceutical Sciences,Central South University,Changsha 410013;3.Second Xiangya Hospital,Central South University,Changsha 410011) |
| Abstract: | Objective To compare anthrone sulfuric acid method and phenol sulfuric acid method in the content determination of polysaccharide in rhizoma of Panax japonicus.Methods The chromogenic conditions and methodology of anthrone sulfuric acid method and phenol sulfuric acid method were compared.Results The content of anthrone sulphuric acid method was higher than that of phenol sulfuric acid method,and the difference was significant.The linearity,reproducibility and stability of anthrone sulfuric acid method were better than those of phenol sulfuric acid method.Conclusion Anthrone sulfuric acid method is better than phenol sulfuric acid method in the content determination of polysaccharide in rhizoma of Panax japonicus. |
| Keywords: | polysaccharide rhizoma of Panax japonicus anthrone sulfuric acid method phenol sulfuric acid method content determination |
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