原发性肝癌组织中三种凋亡相关基因的表达及意义

目的探讨原发性肝癌组织中三种凋亡相关基因：XIAP、Smac及Caspases-3的表达及意义。方法应用免疫组化方法检测67例原发性肝癌组织中XIAP、Smac及Caspases-3的表达情况。结果XIAP、Smac及Caspases-3在正常肝组织中的阳性率分别为（5．9±1．0）％、（90．1±0．8）％和（87．7±1．1）％，在肝癌组织中的阳性率分别为（89．7±0．7）％、（53．21±1．3）％和（49．8±0．9）％，三者在不同性质组织问差异均有统计学意义（t=7．78，5．37，6．01，P〈0．05）；肝癌组织中XIAP与Caspases-3的表达呈负相关（r=-0．69，P〈0．05），Smac与Caspases-3的表达呈正相关（r=0．67，P〈0．05）。结论XIAP、Smac通过影响Caspases-3的表达拮抗细胞凋亡过程，参与原发性肝癌的发生、发展。

The meaning of the expression of three proapoptosis genes in primary hepatic carcinoma

Objective To investigate the meaning of the expression of three proapoptosis genes.XIAP,Smac and caspases-3,in primary hepatic carcinoma.Method The expression of XIAP,Smac and Caspases-3 of 67 cases primary hepatic cancer was detected with immunohistochemistry.Result The posi-tive rate of XIAP,Smac and easpases-3 in tissues of hepatic carcinoma was(89.7±0.7)%,(53.21±1.3)%and(49.8±0.9)%respectively,while(5.9±1.0)%,(90.1±0.8)%and(87.7±1.1)%in normal hepatic tissues.And it had statistical significance(t=7.78,5.37,6.01,P<0.05).This expres-sion of caspases-3 negatively correlated with XIAP(r=-0.69,P<0.05)while positively with Smac(r =0.67,P<0.05).Conclusion XIAP and Smac play a role of anti-apoptosis by effecting the expression of easpases-3 and participate the genesis and development of the cancer.