玻璃化解冻和慢速解冻在程序化冷冻胚胎解冻移植中的比较研究

目的 探讨玻璃化解冻(vitrificative thawing,VT)和慢速解冻(slow thawing,ST)对程序化冷冻胚胎解冻移植的影响,优化程序化冷冻胚胎的解冻方案,从而提高不孕不育临床治疗效果.方法 选取2016年1月至2018年12月解放军第九六〇医院生殖医学中心行解冻胚胎移植的400例患者,使用随机数...

Comparative study of vitrification and slow thawing in programmed frozen embryo thawing transfer

Objective To discuss the influence of vitrificative thawing(VT)and slow thawing(ST)on programmed frozen embryo thawing transfer,optimize the thawing program of programmed frozen embryo,and improve the clinical treatment effect of infertility.Methods From January 2016 to December 2018,400 patients who underwent thawing embryo transfer at Reproductive Medicine Center,the 960th Hospital of the PLA Joint Logistics Support Force were selected and divided into VT groups(200 cases)and ST group(200 cases)(randomized by Excel data)using random number assignment,compare the basic conditions,embryo development and embryo recovery and transfer situation and clinical pregnancy outcome of the two groups during the initial invitro fertilization-embryo transfer(IVF-ET).Results There were no statistically significant differences between the two groups of patients in basic conditions such as age,years of infertility,number of eggs obtained,gonadotropins(Gn)dosage and basic follicle stimulating hormone(FSH)values(P>0.05).There were no significant difference between the two groups of MII egg rate(metaphase II egg rate),fertilization rate,cleavage rate,high-quality embryo rate,usable embryo rate and frozen embryo rate(P>0.05).However,the embryo recovery complete rate and recovery availability rate(88.34%,97.51%)of the VT group were significantly higher than those of the ST group(72.47%,82.23%),and the differences were statistically significant(P<0.001).There were no statistically significant difference in the number of transfers,the number of embryos transferred and the thickness of the intima on the day of embryo transfer(P>0.05).There was no significant difference in the clinical pregnancy outcome between the two groups(P>0.05).Conclusion VT can significantly improve the recovery effect of programmed frozen embryos and increase the embryo utilization rate of patients with programmed frozen embryos.It can be considered as a better alternative to ST.