孕妇外周血NKT细胞水平与妊娠期肝内胆汁淤积症发病及病情关系

目的:探究孕妇外周血自然杀伤T(NTK)细胞水平与妊娠期肝内胆汁淤积症(ICP)发病及病情严重程度相关性。方法:选择2016年3月1日-2018年3月1日本院就诊的ICP患者52例(ICP患者组),根据ICP严重程度分为轻度ICP组(n=28)和重度ICP组(n=24),同时随机选择孕周相当的健康产前检查孕妇52例(健康孕妇组),比较各组外周血单个核细胞(PBMC)中NKT细胞水平,分析NKT细胞水平与ICP发生发展相关性。结果:ICP患者组外周血PBMC中NKT细胞含量高于健康孕妇组(P<0.05)。对ICP患者组和健康孕妇组外周血PBMC给予不同刺激物培养后,均显示出NKT细胞含量在正常组与空白组无差异(P>0.05),而在植物血凝素(PHA)组、ICP组、正常组依次降低(P<0.05)。重度ICP组外周血PBMC中NKT细胞含量高于轻度ICP组(P<0.05),NKT细胞含量与ICP病情严重程度呈正相关关系(P<0.05)。结论:ICP发生及进展与孕妇外周血NKT细胞水平呈正相关关系,监测NKT细胞水平可为诊断ICP提供新思路。

Correlation between the NKT cell levels in peripheral blood of preganant women and their incidence and severity of intrahepatic cholestasis of pregnancy

Objective:To investigate the relationship between the natural killer T(NTK)cells level in peripheral blood of preganant women and their incidence and severity of intrahepatic cholestasis of pregnancy(ICP).Methods:52 pregnant women with ICP were enrolled and were divided into group A(28 women with mild ICP)and group B(24 women with severe ICP)from March 1,2016 to March 1,2018.Meanwhile,52 healthy pregnant women during the same gestational weeks were randomly selected in group C.The level of NKT cells in peripheral blood mononuclear cells(PBMC)of women were compared among the three groups.The correlation between NKT cell level and ICP occurrence and development was analyzed.Results:The peripheral blood NKT cell level in PBMC of women with ICP was significant higher than that of healthy pregnant women(P<0.05).The peripheral blood PBMC of women in the three groups was cultured by different stimulants,and the NKT cell level had no significant different between the women in group C and the women with their PBMC cultured no stimulants(P>0.05).The NKT cell level of the women with their PBMC cultured by phytohemagglutinin stimulants,the women with ICP,and women in group C had decreased in turn(P<0.05).The peripheral blood NKT cells level in PBMC of women in group B was significant higher than that of women in group A(P<0.05).The NKT cells level of women with ICP was positively correlated with the severity of ICP(P<0.05).Conclusion:The occurrence and progression of ICP is positively correlated with the level of NKT cells in the peripheral blood of pregnant women,so monitoring the level of NKT cells maybe provide a new idea for diagnosing ICP.