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Isolation and characterization of fifteen microsatellite loci in Leopard grouper (Mycteroperca rosacea) via 454 pyrosequencing |
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| Authors: | Alexis M. Jackson Adrian Munguía-Vega Avian S. Lain Simon A. Stokes Aneese J. Williams Giacomo Bernardi |
| Affiliation: | 1. Department of Ecology and Evolutionary Biology, University of California Santa Cruz, 100 Shaffer Road, Santa Cruz, CA, 95060, USA 2. School of Natural Resources and the Environment, University of Arizona, 1131 E. 4th Street, Tucson, AZ, 85721, USA 3. Comunidad y Biodiversidad A.C., Isla del Peruano 215, Lomas de Miramar, Guaymas, Sonora, 85448, Mexico 4. Department of Chemical Engineering, Hampton University, 100 E. Queen Street, Hampton, VA, 23668, USA 5. Department of Molecular, Cellular and Developmental Biology, University of California Santa Cruz, 1156 High Street, Santa Cruz, CA, 95064, USA 6. Department of Environmental and Marine Science, Hampton University, 100 E. Queen Street, Hampton, VA, 23668, USA |
| Abstract: | Leopard grouper (Mycteroperca rosacea) are distributed from Bahía Magdalena, through the Gulf of California and as far south as Jalisco, Mexico. They are listed as Vulnerable on the IUCN Red List due to significant fishing-induced declines over the past decade. Microsatellite loci were isolated by constructing a shotgun genomic library and sequencing using 454 XL + titanium chemistry. We characterized 15 polymorphic loci in 120 samples from three geographic locations. Genotyping yielded 15–60 alleles per locus with observed levels of heterozygosity ranging from 0.575 to 0.975. |
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