Identification of serotype 9 human rotavirus by enzyme-linked immunosorbent assay with monoclonal antibodies. |
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| Authors: | K Midthun J Valdesuso A Z Kapikian Y Hoshino K Y Green |
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| Affiliation: | Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892. |
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| Abstract: | Hybridomas producing monoclonal antibodies to VP7, a major neutralizing protein of serotype 9 rotavirus (strain W161), were prepared. One monoclonal antibody, W161-6A1, was shown to neutralize only serotype 9 rotavirus strains and reacted specifically with serotype 9 rotaviruses in an enzyme-linked immunosorbent assay. The development of an immunoassay for detection of serotype 9 rotaviruses should facilitate epidemiologic studies. |
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