维生素E对二硫化碳致雄性大鼠睾丸组织脂质过氧化的拮抗作用

目的探讨二硫化碳(CS2)对雄性大鼠睾丸组织氧自由基及抗氧化水平的影响,同时观察维生素E(VE)对其的拮抗作用。方法取健康Wistar雄性大鼠36只随机分为6组,以不同浓度CS2(0、50、250、1250mgm3)静式吸入染毒,共10周,另设1250mgm3(CS2)加VE(250mgkg)组和单纯VE(250mgkg)组,VE拌入饲料中,染毒结束后,处死动物取出睾丸制备组织匀浆,分别测定各组SOD、MDA、GST、GSH、GSHpx、NO、总NOS和iNOS水平,同时检测VE对其的拮抗水平。结果睾丸组织中SOD活力下降,MDA含量上升,与对照组比较有显著差异(P<0.05);GSH含量、GST、GSHpx活力总体趋势下降,与对照组比较有显著差异(P<0.05或P<0.01);NO含量及总NOS、iNOS活力均下降,有随染毒浓度增加而降低的趋势,与对照组比较有显著差异(P<0.05或P<0.01)。但用VE干预后,SOD、GST、GSHpx、总NOS、iNOS活力均有不同程度上升,GSH、NO含量亦是如此,而MDA含量则下降。结论VE对CS2致睾丸组织脂质过氧化有拮抗作用。

Antagonism of vitamin E on lipid peroxidation in testis of male rats with carbon disulfide

Objective To study the antagonistic joint action of vitamin E(VE) lipid peroxidation of testis in the male rats with carbon disulfide(CS_2). Methods 36 wistar male rats were randomly dicided into six groups. It took 10-week for the rats to breath CS_2 in different concentrations(0, 50, 250 and 1250mg/m3), respectively,CS_2(1250mg/m3) with VE(250mg/kg diet) and VE(250mg/kg diet)group. After 10-week treatment, rats were killed and the following parameters of lipid peroxidation in testis were determined:SOD, MDA, GST, GSH, GSH-px, NO, NOS and iNOS.Results Compare with the control group, SOD, GST, GSH-px, NOS and iNOS activity were decreased (P<0.05 or P<0.01), GSH, NO content decreased also, MDA content was increased (P<0.01). After the antioxidant VE treatmented, the parameters were significantly increased (the amount of MDA was decreased). Conclusion The antioxidant VE significantly protects against on lipid peroxidation in testes of male rats with CS_2.