人突变的载脂蛋白E对转基因小鼠学习与记忆能力的影响

目的 研究突变的人载脂蛋白Ｅ（ａｐｏＥ）对大脑皮层功能的影响。方法 以Ｆ１代ａｐｏＥ４与ａｐｏＥ７转基因小鼠为研究对象,分别用Ｓｏｕｔｈｅｒｎ印迹分析及ＥＬＩＳＡ检测人ａｐｏＥ基因在Ｆ１代小鼠染色体上的整合与在血中的表达;以主动回避实验检测小鼠的学习能力和短期与长期记忆能力的变化,同时用酶法测定Ｆ１代小鼠的血脂水平。结果 （１）人ａｐｏＥ４和ａｐｏＥ７基因稳定地整合于Ｆ１代小鼠染色体上并高效表达于

The influences of human apolipoprotein E mutants on the learning and memory of cerebral cortex in transgenic mice

OBJECTIVE: To study the influences of the mutants of human apolipoprotein E on the function of cerebral cortex. METHODS: The Southern blot hybridization and ELISA techniques were used for identification of integration and expression of human apoE4 and apoE7 genes at the mice chromosome and in the serum of F1 transgenic mice. Then the active avoidance response test was performed to determine the alteration of learning and short term and long term memory of the transgenic mice. The serum total cholesterol (TC) and triglyceride (TG) were determined simultaneously with enzymatic procedure. RESULTS: (1) In F1 transgenic mice the human apoE genes integrated at the chromosome and expressed in their serum stably. (2) In apoE4 mice the learning and short term memorizing abilities were decreased, and in apoE7 mice the short term memorizing abilities were decreased as well. In addition, the TC and TG increased statistically. CONCLUSIONS: The overexpression of human apoE4 and apoE7 genes in transgenic mice could damage the function of cerebral cortex. It denoted that the mutations of apoE might correlate the pathogenesis of Alzheimer's disease.