同种异体脱钙骨基质复合自体骨髓基质干细胞修复兔关节软骨缺损

背景:脱钙骨基质具有良好的生物相容性,是临床常用的骨缺损修复材料.骨髓基质干细胞具有向骨及软骨细胞分化的潜能,适合骨软骨缺损修复.目的:观察同种异体脱钙骨基质复合自体骨髓基质干细胞修复兔关节软骨缺损的效果.方法:27只兔均建立骨软骨缺损模型,造模后随机分为3组:复合材料组钻孔植入复合自体骨髓基质干细胞培养8 d的脱钙骨基质块,单纯脱钙骨基质组钻孔单纯植入脱钙骨基质块,模型对照组钻孔后不植入任何材料.取材后对修复组织进行大体观察、组织学观察及Ⅱ型胶原免疫组织化学染色鉴定,并参照Wakitani评分标准对修复组织进行评分.结果与结论:植入后12周,复合材料组修复组织呈透明软骨样,表面光滑平坦,与周围软骨及软骨下骨结合良好;单纯脱钙骨基质组有部分软骨样修复;而模型对照组仅有少量纤维性修复.植入后12周,复合材料组、单纯脱钙骨基质组修复组织Ⅱ型胶原免疫组化染色均呈阳性,修复细胞表达Ⅱ型胶原,为软骨细胞;模型对照组呈阴性表达.植入后4,8,12周,复合材料组修复效果评分均显著优于单纯脱钙骨基质组、模型对照组(P<0.01).说明自体骨髓基质干细胞复合同种异体脱钙骨基质支架材料修复兔全层关节软骨缺损的效果良好.

Allograft decalcified bone matrix combined with autologous bone marrow stromal cells for repairing articular cartilage defects in rabbits

BACKGROUND: Demineralized bone matrix (DBM) having a great biocompatibility is a common material to repair bone defect in clinic Bone marrow stromal cells (BMSCs) which can differentiate into bone and cartilage cells are ideal for repairing cartilage defect. OBJECTIVE: To observe the effects of allograft DBM combined with autograft BMSCs on repairing the articular cartilage of rabbits. METHODS: Bone and cartilage defect was induced in 27 rabbits and the models were randomly divided into combined group (DBM combined with 8-day-curtured autograft BMSCs), DBM group (DBM implantation alone), and control group. The reparative tissue samples were evaluated grossly, histologically, and immunohistochemically. The repairing effect was evaluated by Wakitani's score system.RESULTS AND CONCLUSION: The repaired tissues were hyaline cartilage-shaped, smooth and glossy, and well combined with peripheral cartilage and subchondral tissues at 12 weeks after implantation in the combined group; some tissues were cartilage-likely repaired in the DBM group; a few of tissues were fiber-like repaired in the control group. Immunohistochemical staining showed that type Ⅱ collagen was positive in both combined and DBM groups. Repaired cells which could express type Ⅱ collagen were cartilage cells. However, the expression of type Ⅱ collagen was negative in the control group. At 4,8, and 12 weeks after implantation, the scores in the combined group were significantly higher than in the DBM and control groups (P < 0.01), suggesting that DBM/BMSCs complex could be an efficient graft to repair the articular cartilage defects.