非变性Ⅱ型胶原蛋白对老年db/db小鼠骨退行性疾病防治作用

目的 探讨非变性Ⅱ型胶原蛋白(UCⅡ)对老年db/db小鼠骨退行性疾病的防治作用及机制.方法 将终生喂养的db/db小鼠分为老年模型对照组、胶原蛋白组、阳性对照组,同时以db/m和青年db/db小鼠为正常对照组和青年对照组,干预16周后检测血糖水平、骨形态计量学指数、炎症指标和基质金属蛋白酶(MMP)水平.结果 胶原蛋...

Protective effect of nondenatured type 2 collagen on degenerative bone disease in aging db/db mice

Objective To explore the effect and mechanism of undenatured type Ⅱ collogen(UCⅡ)on degenerative bone disease in aging db/db mice.Methods Db/db mice(15 for each group)with lifetime feeding were randomly assigned into an aging model group(without treatment),UCⅡ treatment group(6 mg/kg UCⅡ in drinking water),and positive control group(180 mg/kg chondroitin sulfate and 225 mg/kg glucosamine in drinking water)and the treatments were carried out when the mice being 50 weeks old and continued for 16 weeks;a normal control group(15 db/m mice)and a young control group(15 Db/db mice of 25-week old at the end of experiment)were also established.By the end of the treatments,all the mice's blood glucose,bone histomorphometry index,inflammation index,as well as matrix metalloproteinase(MMP)were determined.Results The fasting blood glucose of UCⅡ-treated mice was significantly lower than that of aging mice and young control mice(both P<0.05).The bone mineral density(BMD)of the mice in UCⅡ treatment group(439.11 mg/cm^3)was significantly higher than those in the aging model group(164.20 mg/cm^3),the normal control group(209.74 mg/cm^3)and the positive control group(294.42 mg/cm^3)(all P<0.05).Significantly increased in the percent of trabecular area and trabecular number were detected in the mice with UCⅡ treatment in contrast to the aging model mice(both P<0.05)and significantly subdued trabecular separation was observed in the mice treated with UCⅡ in comparison with that in the aging model and young control mice(both P<0.05).The serum interleukin 1 beta(IL-1β)and tumor necrosis factor-alpha(TNF-α)in UCⅡ treated mice was significantly lower than those in the aging model mice(both P<0.05).The UCⅡ treated mice had significantly lower serum MMP-1 compared to aging model mice and lower serum MMP-3 compared to aging model,young control,and positive control mice(P<0.05 for all).Conclusion Undenatured type Ⅱ collogen could improve blood sugar level,increase BMD,and alleviate osteoporosis and osteoarthritis in aging db/db mice;these effects may be related to the alleviation of inflammatory responses and the reduction of serum MMPs.