| RP-HPLC法测定红花注射液中腺苷的含量 |
| |
| 引用本文： | 苗爱东,彭燕,王本富,胡辉,黄景欧,李军胜.RP-HPLC法测定红花注射液中腺苷的含量[J].中成药,2003,25(3):199-200. |
| |
| 作者姓名： | 苗爱东 彭燕 王本富 胡辉 黄景欧 李军胜 |
| |
| 作者单位： | 中国人民解放军第474医院,新疆,乌鲁木齐,830011 |
| |
| 摘 要： | 目的 :建立HPLC法测定红花注射液中腺苷的含量。方法 :采用色谱柱 :DiamonsilTM C1 8柱 ,流动相 :甲醇 1 %醋酸(梯度洗脱 ) ,以腺苷为对照品 ,外标法定量 ,紫外检测波长 2 70nm。结果 :腺苷在 5～ 50 μg·mL- 1 范围内呈良好的线性关系。平均回收率 1 0 1 .86 % ,RSD为 1 .34 %。结论 :该方法简便、快速准确 ,可作为该制剂的质量控制方法。
|
| 关 键 词： | 红花注射液 腺苷 HPLC |
| 文章编号： | 1001-1528(2003)03-0199-02 |
| 修稿时间： | 2002年4月24日 |
Determination on adenosin of Safflower Injection by RP-HPLC |
| |
| MIAO Ai dong,PEN Yan,WANG Ben Fu et al.Determination on adenosin of Safflower Injection by RP-HPLC[J].Chinese Traditional Patent Medicine,2003,25(3):199-200. |
| |
| Authors: | MIAO Ai dong PEN Yan WANG Ben Fu |
| |
| Abstract: | Objective: To establish a RP HPLC method to determine the content of adenosin in safflower Injection. Methods: The sample was determined by RP HPLC with a Diamonsil TM C 18 column, UV detection wavelength was at 270nm. adenosin used as an external standard and CH 3OH 1% HAC as a mobile phase. Results: The average recovery was 101.86%, RSD was 1.34%. Conclusion: The method is simple and accurate. It can be used to control the quality of Honghua Injection. |
| |
| Keywords: | Honghua Injection adenosin HPLC determination |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
