黄芪对肺腺癌细胞生长抑制的实验研究 |
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引用本文: | 耿国军 姜杰 杜好信 钱文轩 张义 杨丽华 陈端扬 陈隽鹏. 黄芪对肺腺癌细胞生长抑制的实验研究[J]. 实用中西医结合临床, 2011, 11(1): 4-5. DOI: 10.3969/j.issn.1671-4040.2011.01.002 |
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作者姓名: | 耿国军 姜杰 杜好信 钱文轩 张义 杨丽华 陈端扬 陈隽鹏 |
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作者单位: | 1. 福建省厦门市中医院,厦门,361000 2. 厦门大学医学院,福建厦门,361009 |
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基金项目: | 福建省自然科学基金项目 |
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摘 要: | 目的:研究分化诱导剂黄芪(Astragalus)在体外对人肺腺癌SPC-A-1细胞生长抑制的影响。方法:用不同浓度的黄芪分别处理肺腺癌SPC-A-1细胞后,镜下观察癌细胞的生长情况;测定软琼脂克隆形成率;MTT(噻唑蓝)法测定生长抑制率。结果:不同浓度黄芪处理后细胞的生长明显受到抑制,细胞生长抑制率随黄芪的浓度增加而增加。结论:黄芪对肺腺癌SPC-A-1细胞的生长有明显的抑制作用。
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关 键 词: | 黄芪 肺腺癌 SPC-A-1 MTT法 生长抑制 |
Experimental Study of the Growth Suppression Effects on Human Lung Cancer Cell Lines with Astragalus |
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Abstract: | Objective:To study the activity of the growth suppression effects with Astragalus on SPC-A-1 human lung adenocarcinoma cel1.Methods:Different concentrations of Astragalus were used in the culture of SPC-A-1 cells.Determination of clone forming ability on soft agar and growth suppression effects with Astragalus were measured by a tetrazolium-based volorimetric assay(MTT assay).Results:Proliferation of SPC-A-1 cells was inhibited effectively after being treated by different concentrations of Astragalus,the ra... |
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Keywords: | SPC-A-1 |
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