| OX40-OX40L相互作用对C57BL/6小鼠淋巴细胞活性的影响 |
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| 引用本文： | 苏红玲,严金川,杨海兵,徐良洁. OX40-OX40L相互作用对C57BL/6小鼠淋巴细胞活性的影响[J]. 江苏医药, 2012, 38(8): 878-880 |
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| 作者姓名： | 苏红玲 严金川 杨海兵 徐良洁 |
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| 作者单位： | 212001,江苏大学附属医院心脏病科 |
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| 基金项目： | 江苏省医学重点人才基金,镇江市社会发展项目 |
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| 摘 要： | 目的探讨OX40-OX40L相互作用对C57BL/6小鼠脾淋巴细胞增殖及细胞因子表达的影响。方法应用MTT法检测小鼠淋巴细胞增殖;ELISA法检测淋巴细胞表达细胞因子IL-4、IL-2及INF-γ表达。结果与对照组比较,Anti-OX40特异性刺激OX40-OX40L轴后,小鼠淋巴细胞增殖增强,分泌IL-2和INF-γ含量明显增加,48h细胞增殖最佳;IL-4含量无明显变化。应用特异性anti-OX40L抗体阻断OX40-OX40L轴后,淋巴细胞增殖及分泌细胞因子IL-2和INF-γ含量明显受到抑制。结论 OX40-OX40L相互作用能够调控C57BL/6小鼠脾淋巴细胞增殖及促炎性细胞因子表达。
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| 关 键 词： | OX40 OX40L T细胞 |
Effect of OX40-OX40 ligand interaction on lymphocyte activity in C57BL/6 mice |
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| Affiliation: | SU Hongling,YAN Jinchuan,YANG Haibing,et al.Department of Cardiology,Affiliated Hospital,Jiangsu University,Zhenjiang 212001,CHINA |
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| Abstract: | Objective To investigate the effect of OX40-OX40L interaction on the lymphocyte proliferation and cytokine secretion in C57BL/6 mice.Methods The proliferation of T cell was analyzed by MTT method and the expressions of IL-4,IL-2 and INF-γ in cell culture supernatant were measured by ELISA.Results Compared with the cells without anti-OX40 mAb stimulation,after stimulating OX40-OX40L signal pathway with anti-OX40,the lymphocyte proliferation and the secreations of IL-2 and INF-γ were significantly increased,which reached a maximum at 48 h.IL-4 expression was not significantly changed.Anti-OX40L mAb significantly suppressed the lymphocyte proliferation and cytokine secretion in C57BL/6 mice.Conclusion OX40-OX40L interaction can regulate the lymphocyte proliferation and proinflammatory cytokine secretion in C57BL/6 mice. |
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| Keywords: | OX40 OX40L T cell |
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