| 抑癌基因PTEN与胶质瘤细胞侵袭和迁移的体外实验研究 |
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| 引用本文： | 王虎,浦佩玉,王广秀,康春生,董伦,李捷,王春艳. 抑癌基因PTEN与胶质瘤细胞侵袭和迁移的体外实验研究[J]. 中国临床神经科学, 2005, 13(4): 339-342 |
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| 作者姓名： | 王虎 浦佩玉 王广秀 康春生 董伦 李捷 王春艳 |
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| 作者单位： | 1. 天津市环湖医院,300060
2. 天津医科大学总医院神经外科,300052 |
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| 基金项目： | 国家自然科学基金(No.39970338)资助 |
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| 摘 要： | 目的:研究抑癌基因PTEN对脑胶质瘤细胞侵袭和迁移的影响。方法:用PTEN表达型质粒转染大鼠C6胶质瘤细胞,利用Matrigel体外侵袭实验和细胞迁移运动实验进行细胞侵袭和迁移的研究。结果:转染PTEN的大鼠C6胶质瘤细胞侵袭与迁移能力均明显下降。结论:PTEN在体外可以有效的抑制肿瘤细胞的侵袭和迁移过程,其具体机制还有待进一步探讨。
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| 关 键 词： | 胶质瘤 PTEN 基因转染 侵袭 |
| 文章编号： | 1008-0678(2005)04-0339-04 |
| 修稿时间： | 2005-05-18 |
Inhibitory Effect of PTEN Transfected on Proliferation and Invasion of Gliomas |
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| WANG Hu,PU Pei-Yu,WANG Guang-Xiu,KANG Chun-Sheng,DONG Lun,LI Jie,WANG Chun-Yan. Inhibitory Effect of PTEN Transfected on Proliferation and Invasion of Gliomas[J]. Chinese Journal of Clinical Neurosciences, 2005, 13(4): 339-342 |
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| Authors: | WANG Hu PU Pei-Yu WANG Guang-Xiu KANG Chun-Sheng DONG Lun LI Jie WANG Chun-Yan |
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| Affiliation: | WANG Hu,PU Pei-Yu,WANG Guang-Xiu,KANG Chun-Sheng,DONG Lun,LI Jie,WANG Chun-Yan Tianjin Huanhu Hospital~*,Dept.of Neurosurgery,General Hospital,Tianjin Medical University,Tianjin 300052,China |
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| Abstract: | Aim:To study the inhibition effects of proliferation and invasion of PTEN on growth of C6 glioma cells in vitro.Methods:C6 cells trnsfected with PTEN plasmid,cell invasion and migration were examined by Transwell method and spheroid/wound-healing method,respectively.Results:In PTEN transfected group,C6 cells'proliferation and invasion were inhibited significantly.Conclusion:PTEN plays a critical role in the proliferation and invasion progression of gliomas and be an important candidate for gene therapy of human gliomas. |
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| Keywords: | glioma phosphatase and tensin homology deleted on chromosome 10 gene transfected invasion |
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