| lncRNA CASC2对结直肠癌细胞功能及血管生成的影响 |
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| 引用本文： | 陈函,罗晓勇,李朝萍,郭彩茹,鞠文翠,翟秧秧,师鑫鹏.lncRNA CASC2对结直肠癌细胞功能及血管生成的影响[J].实用肿瘤学杂志,2022,36(2):111-118. |
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| 作者姓名： | 陈函 罗晓勇 李朝萍 郭彩茹 鞠文翠 翟秧秧 师鑫鹏 |
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| 作者单位： | 1.郑州大学第二附属医院(郑州 450014); 2.洛阳市肿瘤临床诊疗研究中心; 3.郑州大学附属洛阳中心医院放疗科; 4.河南省肿瘤免疫与再生医学国际联合实验室 |
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| 基金项目： | 2020年河南省医学科技攻关计划项目(编号:202102310421);2020年度洛阳市医疗卫生领域指导性科技计划项目(编号:2020002A) |
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| 摘 要： | 目的 研究lncRNA CASC2过表达对结直肠癌细胞增殖、迁移、血管生成及Wnt/FZD/β-catenin信号通路的影响.方法 qRT-PCR检测lncRNA CASC2在正常结肠上皮细胞(NCM460)及结直肠癌细胞株(DLD-1、HT29、SW620、HCT116、RKO、LOVO、SW480)中的表达情况;H...
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| 关 键 词： | 长链非编码RNA CASC2 结直肠癌 外泌体 Wnt信号通路 血管生成 |
| 收稿时间： | 2021-09-30 |
The effect of lncRNA CASC2 on the function and angiogenesis of colorectal cancer cells |
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| CHEN Han,LUO Xiaoyong,LI Chaoping,GUO Cairu,JU Wencui,ZHAI Yangyang,SHI Xinpeng.The effect of lncRNA CASC2 on the function and angiogenesis of colorectal cancer cells[J].Journal of Practical Oncology,2022,36(2):111-118. |
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| Authors: | CHEN Han LUO Xiaoyong LI Chaoping GUO Cairu JU Wencui ZHAI Yangyang SHI Xinpeng |
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| Institution: | 1. The Second Affiliated Hospital of Zhengzhou University,Zhengzhou 450014,China; 2. Luoyang Cancer Clinical Diagnosis and Treatment Center; 3. Department of Radiation Oncology,Luoyang Central Hospital Affiliated to Zhengzhou University; 4. Henan International Joint Laboratory of Tumor Immunology and Regenerative Medicine |
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| Abstract: | Objective The of this study was to investigate the effect of lncRNA CASC2 overexpression on the proliferation,migration,angiogenesis and Wnt/FZD/β-catenin signaling pathway of colorectal cancer cells.Methods qRT-PCR was used to detect the expression of lncRNA CASC2 in normal colon epithelial cells(NCM460)and colorectal cancer cell lines(DLD-1,HT29,SW620,HCT116,RKO,LOVO,and SW480).HCT116 cells and SW480 cells were established stable transfection.The transfected cell lines were divided into the control group and lncRNA CASC2 overexpression group.CCK-8,Transwell,and scratch assays were used to detect the cell proliferation and migration ability.The cell supernatants collected from the control group and the lncRNA CASC2 overexpression group were used to extract exosomes,which were incubated with HUVEC cells.Their effects on angiogenesis were detected by 3D Matrigel method.The protein expression of Wnt16,FZD7 and β-catenin were determined by Western blot.Results The expression of lncRNA CASC2 in 7 colorectal cancer cell lines was significantly lower than that in normal colon epithelial NCM460 cells.Compared with the control group,the overexpression of lncRNA CASC2 significantly inhibited the proliferation,migration of HCT116 cells and SW480 cells(P＜0.01).3D Matrigel assay showed that exosomes from cells in SW480-CASC2 and HCT116-CASC2 groups significantly inhibited the formation of tubular cells in HUVEC(P＜0.01).The results of Western blot showed that the levels of Wnt16,FZD7 and β-catenin protein decreased after lncRNA CASC2 overexpression(P＜0.01).Conclusion The expression of lncRNA CASC2 is down-regulated in colorectal cancer cells.Overexpression of lncRNA CASC2 can inhibit the proliferation and migration of colorectal cancer cells by inhibiting the Wnt/FZD/β-catenin signaling pathway,and reducing angiogenesis. |
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| Keywords: | Long non-coding RNA CASC2 Colorectal cancer Exosomes Wnt signaling pathway Angiogenesis |
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