| RP-HPLC法测定人血浆中穿琥宁含量及药代动力学研究 |
| |
| 引用本文： | 曹露晔,郭莹,张阳儿,颜仁梁,闫晶超.RP-HPLC法测定人血浆中穿琥宁含量及药代动力学研究[J].药物分析杂志,2012(4):721-724. |
| |
| 作者姓名： | 曹露晔 郭莹 张阳儿 颜仁梁 闫晶超 |
| |
| 作者单位： | 广东食品药品职业学院;浙江中医药大学生物工程学院;上海市五官科医院临床药理室 |
| |
| 基金项目： | 浙江省中医药管理局项目(2010ZQ002);浙江省卫生厅项目(2010KYB076) |
| |
| 摘 要： | 目的:研究穿琥宁在人体内的药代动力学过程并建立人生物样品的高效液相色谱方法。方法:采用液-液萃取的方法从生物样品中提取出穿琥宁后用Diaminsil-C18(2)色谱柱(250 mm×4.6 mm,5μm)柱进行分离,采用的流动相为:乙腈-磷酸盐缓冲液0.5 g.L-1磷酸二氢钾,用磷酸调节pH至2.5(±0.05)](43∶57,v/v)。结果:穿琥宁在人体内静脉给药200 mg后药代动力学符合二室模型,统计距参数为:Cmax为(9.796±2.306)μg.mL-1、Tmax(1.382±0.231)h、T1/2z(1.678±0.509)h。结论:此方法快捷、灵敏、简便,适合于穿琥宁的药代动力学研究。
|
| 关 键 词： | 穿琥宁 反相高效液相色谱 药代动力学 |
RP-HPLC determination and its pharmacokinetic research of dehydroandrograpolide succinate sodium for injection in human plasma |
| |
| CAO Lu-ye,GUO Ying,ZHANG Yang-er,YAN Ren-liang,YAN Jing-chao.RP-HPLC determination and its pharmacokinetic research of dehydroandrograpolide succinate sodium for injection in human plasma[J].Chinese Journal of Pharmaceutical Analysis,2012(4):721-724. |
| |
| Authors: | CAO Lu-ye GUO Ying ZHANG Yang-er YAN Ren-liang YAN Jing-chao |
| |
| Institution: | 1.Food and Drug vocational College of Guangdong,Guangzhou 510520,China; 2.College of Bio-engineering,Zhejiang Chinese Medical University,Hangzhou 310053,China; 3.Institute of Clinical Pharmacology,Shanghai Facial Features Hospital,Shanghai 201203,China) |
| |
| Abstract: | Objective:To study the pharmacokinetic of dehydroandrograpolide succinate sodium for injection in human and establish the HPLC method in human plasma.Methods:A validated HPLC method is described for the determination of dehydroandrograpolide succinate sodium for injection in human plasma using a liquid-liquid extraction and ultraviolet(UV) absorbance detection.Result:The extraction recovery of dehydroandrograpolide succinate sodium for injection ranged from 70.040% to 79.465%.The linear range was 0.05-100 μg·mL-1,correlation coefficient r2=0.998.Dehydroandrograpolide succinate sodium for injection pharmacokinetic application with intravenous it in human revealed that dehydroandrograpolide succinate sodium for injection followed two-compartment open model.Cmax/μg·mL-1:9.796±2.306、Tmax/ h :1.382±0.231、T1/2z/h:1.678± 0.509.Conclusion:The method developed was successfully applied to a simple,rapid,specific and accurate HPLC method for investigation of the pharmacokinetics of dehydroandrograpolide succinate sodium for injection in human. |
| |
| Keywords: | dehydroandrograpolide succinate sodium for injection RP-HPLC pharmacokinetic |
| 本文献已被 CNKI 等数据库收录！ |
