台州地区汉族哮喘人群5-HTT基因Stin2位点多态性分析与抑郁易感性研究

<正>支气管哮喘(简称哮喘)是气道慢性炎症疾病,也是具有多基因遗传倾向的疾病。5-羟色胺(5-hydroxytryptamine,5-HT)作为一种广泛存在的抑制性神经递质,参与情绪神经活动的调节,与抑郁发生密切相关,亦是哮喘神经因素发生机制的重要组成部分。5-HTT的第二内含子可变数目串联重复序列(variable number of tandem repeats,VNTR)多态性即Stin2位

Polymorphism analysis of Stin2 locus of 5-HTT gene and susceptibility to depression in Han population of asthma in Taizhou areaLIN Ling,XU Youwen,XU Jiajia,PEI Lu,LV Dongqing. Department of Respiratory Medicine, Taizhou Hospital of Wenzhou Medical University, Linhai, Zhejiang province,317000,China

Objective To investigate the association between serotonin transporter (5-HTT) gene polymorphism and asthma and asthma with depression in Han nationality in Taizhou. Methods Polymerase chain reaction (PCR) was used to detect the genetic polymorphisms of the Stin2 locus in 204 confirmed asthma patients and 200 healthy subjects, and to explore the molecular genetic mechanism of asthma. Depression was assessed by the Self-rating Depression Scale (SDS). The asthma group and healthy components were included in the depression group and the non-depression group. The depression in the asthma group was analyzed. Further analysis of the different genotypes and allele frequencies of Stin2 was associated with depression in the asthma-compressed group. Results The frequencies of Stin2.10/12 genotype and Stin2.10 allele in the asthma group were (25.49, 15.84%) and the healthy group were statistically different (P<0.05). 88 patients with asthma complicated with depression, the incidence rate was 43.1%, and the incidence of concurrent depression in the healthy control group was 17%. There were significant differences in the expression of Stin2.10/Stin2.10, Stin2.10/Stin2.12, Stin2.12/Stin2.12 genotypes and asthma non-depression group in asthma group (P<0.01). The expression of the 12 and 10 alleles of the Stin2 gene in the asthma group was significantly different between the non-depressed group and the non-depressed group (P<0.05). Conclusion The Stin2 polymorphism of 5-HTT gene may be associated with asthma in Han population in Taizhou, and it is a related gene for depression in asthmatic population.