高通量测序技术分析喂养不耐受早产儿肠道菌群的动态变化

目的:了解喂养不耐受(FI)早产儿肠道菌群的动态变化。方法:选取2017年1-7月徐州医科大学附属徐州市妇幼保健院新生儿科收治的56例喂养不耐受早产儿,入院后常规应用广谱青霉素,发生喂养不耐受时给予小剂量红霉素促进胃肠动力治疗。采集患儿生后首次胎便(FI1组)、出现喂养不耐受时的粪便(FI2组)、喂养不耐受消失后的粪便(FI3组),采用高通量测序技术对粪便样本中所有细菌进行16S rRNA测序,比较各组菌群的多样性及丰度变化。结果:与FI1组相比,FI2组的Shannon、Ace指数均下降,克雷伯杆菌丰度升高,拟杆菌、双歧杆菌、乳酸杆菌、罗尔斯通菌、红球菌的丰度降低(P均<0.05)。FI3组与FI2组的Shannon、Ace指数及克雷伯杆菌、双歧杆菌、乳酸杆菌、红球菌丰度比较差异均无统计学意义(P均>0.05),FI3组的拟杆菌、罗尔斯通菌丰度继续降低(P均<0.05)。结论:早产儿喂养不耐受发生时,肠道菌群的多样性及丰度明显降低,克雷伯杆菌丰度升高,拟杆菌、双歧杆菌、乳酸杆菌、罗尔斯通菌、红球菌的丰度降低。

Dynamic Changes of Gut Microbiota in Preterm Infants with Feeding Intolerance by High-ThroughputSequencing Technology

Objective: To investigate the dynamic changes of gut microbiota in preterm infants with feeding intolerance(FI) by high-throughput sequencing technology. Methods: A total of 56 preterm infants with feeding intolerance admitted into Xuzhou Maternal and Child Health Hospital Affiliated to Xuzhou Medical University from Jan. to Jul. 2017 were extracted. After admission, broad-spectrum penicillin was used empirically, and small dose erythromycin was given to promote gastrointestinal motility after feeding intolerance. The first meconium (FI1 group) after birth, the feces after feeding intolerance (FI2 group), and the feces after disappearance of feeding intolerance (FI3 group) were collected. 16S rRNA sequencing was performed on all the bacteria in the feces samples by using high-throughput technology. The diversity and abundance of each group were compared. Results: Compared with the FI1 group, the Shannon and Ace indexes of the FI2 group decreased, the abundance of Klebsiella increased, and the abundance of Bacteroides, Bifidobacteria,Lactobacillus, Rollstonella, and Rhodococcus decreased (P<0.05). There was no significant difference in the Shannon, Ace indexes and the abundance of Klebsiella, Bifidobacteria, Lactobacillus and Rollstonella between the FI3 group and the FI2 group (P>0.05), while the abundance of Bacteroides and Rolestella in FI3 group was in a decreasing trend (P<0.05). Conclusion: The diversity and abundance of gut microbiota in preterm infants with feeding intolerance decreased significantly, and the abundance of Klebsiella increased, the abundance of Bacteroides, Bifidobacteria, Lactobacillus, Rollstonella, and Rhodococcus decreased.